little is known about the cells that make up the human liver and its immune microenvironment. Here we report a map of the cellular landscape of the human liver using single-cell RNA sequencing. We provide the transcriptional profiles of 8444 parenchymal and non-parenchymal...
因为TCR经常被用作识别T细胞祖先的独特识别因子,作者利用5个HBV阳性肝癌患者的单细胞RNA-seq数据,通过TraCeR方法收集的全长TCR α链和β链的序列,用来追踪单个T细胞的族系。作者一共检测了94%的T细胞(3,792/4,032)的全部TCR序列,它们都至少有1个配对的生产性TCR α-β链可用于T细胞克隆分析,结果表明TCR序列信息...
Single cell RNA-seqrevealed landscape of infiltrating T cells in HBVliver cancerRNASubject Code:H16With the support by the National Natural Science Foundation of China,a collaborative study by the research groups led by Prof.Zhang Zemin(张泽民)from the Biodynamic Optical Imaging Center(BIOPIC),...
Single-cell RNA sequencing (scRNA-seq) has emerged as a potent approach to exploring cancer and its microenvironment in a single-cell resolution21,22,23,24,25,26but with a deficiency in a morphological resolution for exploring solid tumors. Spatial transcriptomics (ST), a lately-developed high-...
Single-cell RNA-seq reveals the role of pathogenic cell populations in development and progression of chronic diseases. In order to expand our knowledge on cellular heterogeneity, we have developed a single-nucleus RNA-seq2 method tailored for the compre
MAIT mucosal-associated invariant T cells PLC primary liver cancer scRNA-seq single-cell RNA sequencing TAMs tumour-associated macrophages TME tumour microenvironment Tregs regulatory T cells Sorry, something went wrong. Please try again and make sure cookies are enabledCited by (0)Author...
And then the cellular atlas of RILI liver was generated by profiling 9,641 cells isolated from X-ray irradiated mice livers and control ones from RILI mice model using single-cell RNA sequencing (scRNA-seq). Seven cell types were identified, including B cells, natural killer cells, T ...
Liver cancer arises from the evolutionary selection of the dynamic tumor microenvironment (TME), in which the tumor cell generally becomes more heterogeneous; however, the mechanisms of TME-mediated transcriptional diversity of liver cancer remain unclear. Here, we assess transcriptional diversity in 15...
While this field is moving forward, a variety of problems still need to be addressed, including sensitivity, limited capacity to obtain exact single-cell information, data processing methods, as well as others. Methods like single-cell RNA sequencing (scRNA-seq) are usually used together with ...
Paediatric liver cancer is rare, and often associated with a predisposition syndrome. Here, the authors show that 11p15.5 mosaic alteration in the liver is a pre-neoplastic lesion associated with hepatoblastoma, and spatial transcriptomics together with single-nucleus RNAseq identify a an altered zonat...