Untargeted or discovery lipidomics: LC-MS workflow Another untargeted workflow, which is also called discovery lipidomics, involves using chromatographic separations (HPLC) connected to mass spectrometry to compare the lipidome between control...
SCIEX LC-MS/MS solutions empower researchers to delve into the lipidome to meticulously characterize subtle distinctions between lipid species and screen similar compounds with enhanced clarity and confidence. Our mass spectrometry portfolio provides powerful tools that deliver precise and comprehensive insigh...
And LC-MS/MS based lipidomic analysiswas used to explore the characteristic placental lipids in mild and severe ICP. Results: Fourty-four lipids were differentially expressed both in mild and severe ICP placenta. The pathway analysis revealed these lipids are mainly enriched in...
Multiplelipidomicsmethods for targeted and non-targeted approaches — shotgun, LC, MS and MS/MS — necessitate the use of multiple software programs to automate data analysis. There is no informatics suite that supports automated data analysis of all the above mentioned methods.SimLipid softwarehas...
HTortimsTOF fleX. PASEF®acquires significantly more MS/MS data in a single run than traditional methods, translating to over 10x more spectra. This means deeper exploration of your lipidome, uncovering even low-abundance species. With PASEF®technology, even the smallest LC peaks reveal their...
Quantification of Endogenous Endocannabinoids by LC-MS/MS Mesut Bilgin, Andrej Shevchenko Pages 99-107 Lipid Profiling by Supercritical Fluid Chromatography/Mass Spectrometry Takayuki Yamada, Takeshi Bamba Pages 109-131 Mass Spectrometric Analysis of Lipid Hydroperoxides ...
Bile acids, a large family of steroids, have a carboxyl group in the side chain. We have developed a sensitive and rapid method for the analysis of the 66 bile acids samples by LC-MS. Read More Popular Services Host Cell Protein Analysis ...
However, this technology has been replaced by the interfacing of desorption ionization techniques of atmospheric pressure ionization (API) and matrix-assisted laser desorption/ionization (MALDI) with MS. API, usually electro- spray ionization (ESI), is often linked to a liquid chromatography (LC) ...
分离与检测技术:液相色谱-质谱联用(LC-MS)实现高灵敏度脂质分离;“鸟枪法”脂质组学直接分析脂质混合物。 数据分析工具:LipidSearch、LIPID MAPS等软件用于质谱数据解析,结合数据库匹配脂质结构。 样本处理优化:改进脂质提取方案(如甲基叔丁基醚法),减少基质干扰。 4. 多领域应用价值 疾病机制...
在脂质代谢组学研究中,提取脂质和分离外泌体是两个关键步骤,下面为您提供了这两个步骤的一些常用技术方法,希望对您有所帮助。 一、提取脂质 常用方法有Folch和Bligh-Dyer两种: 1.Folch方法: 将样本与氯仿和甲醇的混合溶剂(比例为2:1,v/v)充分混合。