细胞ROS和脂质过氧化检测(Cellular ROS and and lipid peroxidation detection) 细胞于处理前12 h接种于12孔板,用2 μM fer-1(S7243, Selleckchem, USA)或32 μg mL−1 CHDV预处理24 h。细胞ROS的检测是基于DCFH-DA (BL714A, Biosharp, China)的过氧化物依赖氧化形成一种名为二氯荧光素(DCF)的荧光...
Farmer EE, Mueller MJ (2013) ROS-mediated lipid peroxidation and RES- activated signaling. Annu Rev Plant Biol 64: 429-450Farmer EE, Mueller MJ. ROS-mediated lipid peroxidation and RES-activated signaling. Annu Rev Plant Biol. 2013;64:429-50....
osteoporosis by impairing osteoblast function, but its precise mechanisms remain unclear. Here, the authors show that estrogen deficiency reduces GPX4 expression, leading to phospholipid peroxidation that impairs osteoblastogenesis, while GPX4 activators including 6- and 8-Gingerols can mitigate these ...
The objective of this study was to determine the specific relationship between brain aging and changes in the level of oxidative stress, lipid peroxidation (LPO) and in the activities of antioxidant enzymes. We used four different age groups (2-3 months, 10-11 months, 16-17 months and 20-...
Recent evidence suggests that during stress, both lipid peroxidation and RES generation can benefit cells. New results from genetic approaches support a model in which entire membranes can act as supramolecular sinks for singlet oxygen, the predominant reactive oxygen species (ROS) in plastids. RES ...
Lipid peroxidationGlutathioneSuperoxide dismutaseGlutathione peroxidaseAgingRatsLiverBrainMitochondria are especially important in cellular senescence since reactive oxygen species (ROS) have been found to be generated constantly as an endogen threat. On the other hand, mitochondrial defence depends mainly on ...
However, excessively elevated lipid metabolism can lead to lipid peroxidation and ROS accumulation within the cell, further impairing their anti-tumor effects [161] (Fig. 4a). Fig. 4 Mechanism of lipid metabolic reprogramming in immune cells. A hypoxic, glucose-deficient, lipid-rich TME often ...
Because of their abundance of reactive hydrogens, polyunsaturated fatty acids are highly susceptible to lipid peroxidation, which compromises the integrity and function of the cell membrane in which they reside. Latest Research and Reviews ER-mitochondria contacts mediate lipid radical transfer via RMDN3...
However, similar to plants and mammalian cells, Chlamydomonas have also been found to exhibit defined characteristics of ferroptotic cell death during heat stress, which includes the depletion of the glutathione–ascorbate redox system [11], ROS accumulation, and lipid peroxidation [12]. However, ...
Hypoxia and oxidative stress are implicated to RA development and subsequent ROS-mediated cell death which is a critical feature for RA progression. As for cell death and lipid peroxidation, ferroptosis is a newly discovered, iron-dependent, and non-apoptotic cell death which draws various attention...