网络释义 1. 光片照明显微镜 光片照明显微镜(light-sheet microscopy) ☆ 采用光片照明技术,无需对样品切片 -成像深度可达1 cm,可观察超厚样品 ☆超… www.qd-china.com|基于3个网页
使用激光片层扫描显微系统(Light Sheet Fluorescence Microscopy)对大型样品长时间成像 一套成像系统,既能提供大型样品的光学切片,又几乎无光毒性或光漂白性,同时还有非常高的时间分辨率。 蔡司Lightsheet Z.1 恰恰能实现这一切。出众的多视角激光片层扫描显微成像系统(Light Sheet Microscopy)能够记录大型活体样品的发育...
1.光片扫描显微成像技术的原理 光片扫描显微成像技术是继Confocal和双光子技术之后,生物医学领域最流行的成像技术。可以对完整器官进行快速的3D扫描成像。 2.光片扫描显微成像技术的优势 传统的Confocal等扫描方式,是对聚焦点进行扫描,从点扫描到...
(Light-sheet microscopy, SPIM) 唯一一款平面光束激发荧光显微镜 -保证了快速高分辨率的成像 唯一一款超大工作距离荧光显微镜 -实现最大尺寸样品的成像 唯一一款选择性照明荧光显微镜 -大大降低了光漂白和光损伤 工作原理 LaVision BioTec的Ultramicroscope显微镜使用一层光束从样品侧面激发荧光样品,通过CCD来检测成像,而入...
Light-Sheet Microscopy FAQ Answers to the questions we are asked most often. Read More What is Light-Sheet Microscopy? Learn about the fundamentals of the light-sheet microscopy technique. Read More Why Choose Light-Sheet? Comparison of light-sheet, confocal laser scanning and spinning disk co...
Light sheet microscopy (LSM) has emerged as one of most profound three dimensional (3D) imaging tools in the life sciences over the last decade. However, LSM is currently performed with fluorescence detection on one- or multi-photon excitation. Label-free LSM imaging approaches have been rather...
Light-sheet microscopy uses a thin plane of light to illuminate a sample orthogonally to the detection objective such that the axial resolution of the microscope is determined wholly or partly by the thickness of the light sheet. It allows fast volumetric imaging with reduced sample irradiation comp...
An introduction to light-sheet microscopy, an imaging technique where a sheet of laser light illuminates only a thin slice of live or cleared samples.
Benchtop mesoSPIM: a next-generation open-source light-sheet microscope for cleared samples The demand to image large biological samples at high resolution requires improvement in current light-sheet microscopy tools. Here, the authors present an improved, benchtop mesoSPIM with a significantly increased...
This chapter introduces the concept of light sheet microscopy along with practical advice on how to design and build such an instrument. Selective plane illumination microscopy is presented as an alternative to confocal microscopy due to several superior features such as high-speed full-frame acquisitio...