flow cytometryZAP-70 has become one of the most studied prognostic markers in Chronic Lymphocytic Leukemia (CLL). ZAP-70 is remarkable in many ways: ZAP-70 has been identified as the best discriminating gene between prognostically distinct CLL subtypes using large scale gene expression profiling; ...
The major advantage of using immune markers by flow cytometry is the identification of particular leukemia subtype, not recognized by morphologic criteria, which may have prognostic significance. Current literature suggests when peripheral blood (PB) is consisted of 30% blasts or higher diagnosis of ...
The exact elucidation of how LSCs function in the resistance to therapeutic approach has yet to be determined; nevertheless, the potency of single-cell sequencing in ascertaining the heterogeneity and corroborating specific markers in LSCs has been delineated in these investigations. Revealing drug effect...
Here, we outline a protocol to detect simultaneous expression of several putative LSC markers (CD34, CD38, CD45RA, CD90) on primary blast cells of human AML by multiparametric flow cytometry. Furthermore, we show how to quantify three progenitor populations and a putative LSC population with ...
Multicolor flow cytometric (MFC)-based T-ALL MRD reports are limited and traditionally based on the utilization of markers-of-immaturity like TdT and CD99. Moreover, studies demonstrating the multicolor flow cytometric (MFC) approach for the assessment of T-ALL MRD are sparse. Herein, we ...
ZAP-70 has become one of the most studied prognostic markers in Chronic Lymphocytic Leukemia (CLL). ZAP-70 is remarkable in many ways: ZAP-70 has been identified as the best discriminating gene between prognostically distinct CLL subtypes using large scale gene expression profiling; ZAP-70 has ...
In conclusion, we present the PLA-flow method, serving to detect and enumerate cells expressing the BCR-ABL1 fusion protein via flow cytometry, also enabling simultaneous immunostaining of other markers. Considerably larger patient series, including serial samples, will need to be analyzed to evaluate...
flow cytometry in the diagnosis and classification of acute myeloid leukemia and detection of minimal residual lesions, raising our concern about the deficiency from the perspective of immunophenotyping markers due to their high degree of cross reactivity and low specificity among M0-M7 types, and ...
Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up. J. Vis. Exp. (133), e56976, doi:10.3791/56976 (2018). Download Citation Reprints and Permissions JoVE Privacy ...
By flow cytometry and an extensive set of markers, we characterized leukemic cells from the blood and bone marrow of 68 symptomatic patients with hairy cell leukemia (HCL). Hairy cells identified in the large cell gate always expressed CD19, CD20, HLA-DR, CD45RA, and B-ly 7. Other marke...