The unique attributes of flow cytometry therefore make it a valuable technique in the diagnosis and classification of lymphomas as well as the assessment of prognostic markers in lymphoma patients.doi:10.1016/S1521-6926(03)00068-9Maryalice Stetler-Stevenson...
For accurate determination of the immunophenotype, antibodies against lymphocyte markers are applied to tissue sections (immunohistochemistry), cytologic specimens (immunocytochemistry), or individual cells in a fluid medium (flow cytometry). Flow cytometric evaluation of cells from needle aspirates also is...
The blasts express the T cell markers CD3, CD5 and CD7. The B cell marker CD19 is absent. HLA Dr is characteristically negative in T ALL. In this case there is a monoclonal population of CD4. CD8 is absent. Other relevant tests Cytochemistry: Acid phophatase is frequently positive and...
Flow cytometry was performed two days after shRNA transduction to determine the initial GFP-positive proportion of live cells for each shRNA. Subsequently, cells were cultured with doxycyline (40 ng/ml) to induce shRNA expression, and the proportion of GFP-positive cell was measured at the...
Differentiating between Burkitt lymphoma and CD10+ diffuse large B-cell lymphoma: the role of commonly used flow cytometry cell markers and the application... The goal of this study was to evaluate routine flow cytometric (FC) immunophenotypic markers in differentiating between Burkitt lymphoma (BL...
There was also an increase in T6+ cells, Langerhans cell markers in the skin. HLA-Dr + , T6 cells increased in all cases tested, indicative of dendritic cells. These data showed a modification of T lymphocytes only in skin infiltrate of lymphoma. 展开 ...
flow cytometric phenotyping and histopathological WHO classification, and, occasionally, PCR for antigen receptor gene rearrangement (PARR). In canine patients, advanced directed therapeutic interventions is hampered by limited detailed information on molecular markers for dysregulated pathways, tumor character...
Flow cytometry analysis revealed B-cell aplasia in patients who experienced an objective response and effective CAR T-cell growth during the initial evaluation [82, 119]. In contrast, during the course of the experiment, B-cell aplasia was absent in every patient who did not have a response....
(Fig.1b). Cell-type annotation was performed by analysing the expression levels of canonical gene markers (Fig.1c) and differentially expressed genes (DEGs) (Fig.1dand Supplementary Table3). Clustered NHCs were uniformly distributed across patients, cohorts, sample collection sites and patient age...
Thelymphoma cellsexpress mature B-cell markers (CD20, CD79a, Pax5) and IgM (seeFigure 6-3,BandC).IgDis expressed in a subset of cases. Clonal surface light chain is expressed, and light chain restriction often can be demonstrated in the cytoplasm of neoplastic cells on tissue sections byim...