flow cytometrydiagnosisperipheral bloodBackground Recent investigations in chronic myeloid leukemia (CML) have focused on the identification and characterization of leukemic stem cells (LSCs). These cells reside within the CD34+/CD38─/Lin─ fraction and score positive for CD26 (dipeptidylpeptidase IV)...
Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up. J. Vis. Exp. (133), e56976, doi:10.3791/56976 (2018). Download Citation Reprints and Permissions JoVE Privacy ...
Immunophenotyping of acute leukemia: Utility of CD45 for blast cell identification Flow cytometry (FCM) is widely used to phenotype cases of acute leukemia and is important in their classification. When bone marrow (BM) is largely infiltr... JP Vial,F Lacombe - 《Methods in Cell Biology》 被...
oligonucleotides. Finally, the fluorescence intensity of individual cells is measured by flow cytometry. We demonstrate that PLA-flow is a rapid, sensitive and specific method with results that correlate well with those of RQ-PCR for the fusion transcript. In addition, usage of flow cytometry gives...
Acute myeloid leukemia (AML) is a heterogeneous, and if not treated, fatal disease. It is the most common cause of leukemia-associated mortality in adults. Initially, AML is a disease of hematopoietic stem cells (HSC) characterized by arrest of differentiation, subsequent accumulation of leukemia...
Flow cytometric analyses of bone marrow cellular DNA content were performed in 115 adult patients with acute leukemia to assess whether and how proliferative activity relates to other disease and host parameters, to antileukemic effect of induction chemotherapy, and to patient prognosis. Eighty ...
Early diagnosis and risk stratification are key to improve outcomes in light-chain (AL) amyloidosis. Here we used multidimensional-flow-cytometry (MFC) to characterize bone marrow (BM) plasma cells (PCs) from a series of 166 patients including newly-diag
Background Acute myeloid leukemia (AML) minimal/measurable residual disease (MRD) by multicolor flow cytometry is a complex laboratory developed test (LDT), challenging for implementation. We share our experience in the validation of a 12‐color AML MRD flow cytometry assay to meet stringent regulat...
2007. Flow cytometry analysis of acute promyelocytic leukemia: the power of 'surface hematology'. Leukemia 21(1):4-8.DI NOTO R,MIRABELLI P,DEL VECCHIO L.Flow cyto metry analysis of acute promyelocytic leukemia:the power of'surface hematology'[J].Leukemia,2007,21(1):4-8....
A single-tube eight-color multiparametric flow cytometry (MFC) panel has good sensitivity for minimal/measurable residual disease (MRD) and excellent prediction for survival among patients with B-cell acute lymphoblastic leukemia (B-ALL), according to a