The protocol to be chosen will depend on the quality and quantity of DNA needed, the nature of samples, and the presence of natural substances that may interfere with the extraction and subsequent analysis. The protocol described in this chapter has been tested for extracting DNA from eight ...
The extracted DNA was successfully amplified by PCR using arbitrary RAPD primers. The same protocol will probably be useful for extraction of high-molecular weight DNA from other plant materials containing large amounts of secondary metabolites and essential oils. 展开 ...
or modifying their functions in a controlled manner [12]. Additionally, the infiltration-centrifugation method can be used to the extraction of AWF added to media prepared for endophyte microorganism cultured in vitro [2,12]. Hong et al. emphasized the need for a detailed understanding of the m...
R. Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components. Plant Mol. Biol. Rep. 15, 8–15 (1997). Article CAS Google Scholar Li, H. Minimap2: pairwise alignment for nucleotide sequences. Bioinformatics 34, 3094–3100 (2018). ...
DNA extraction, PCR amplification, and sequencing DNA was extracted from mycelia grown on MEA plates with CTAB plant genome DNA fast extraction kit (Aidlab Biotechnologies Co., Ltd, Beijing, China). The ITS region was amplified with the primers ITS1 and ITS4 (White et al. 1990), the tub...
a PowerSoil DNA Isolation Kit following the manufacturer’s instructions (MoBio, Carlsbad, CA, USA). DNA extraction from willow leaves and roots (100 mg per sample) was performed using a MoBioPowerPlant Pro DNA Isolation Kit (Mo Bio Laboratories) according to the manufacturer’s protocol. ...
DNA extraction, library construction, and genome sequencing Fresh and healthy leaves were promptly collected and flash-frozen in liquid nitrogen. Subsequently, these frozen samples were sent to the biotechnology company BioMarker with dry ice during transit. High-quality genomic DNA was extracted from ...
Papaya Sex-Linked DNA Markers and Traits Genes for Sex-Determination and Fruit Quality Carica papaya is a triecious species comprising individuals of male, hermaphrodite, and female sexes; the plants are diploid (2n=18). The male plants are solely pollinators and economically unimportant, a...
ROC5 was initially identified in a T-DNA insertion mutant called outcurved leaf1 (oul1), which exhibited a distinctive abaxially rolled leaves that was attributable to an elevated number and enlarged size of bulliform cells. Subsequently, the leaves of ROC5 overexpressing plants displayed a ...
The MBP-MPL1 protein was purified from the crude extract by amylose resin affinity chromatography (E8201S, New England Biolabs, USA) following the manufacturer’s protocol. For the EMSAs, the probes were prepared by amplifying PCR products from the MPL1 promoter using Primestar HS DNA Polymerase...