目的 筛选一种操作简单、快捷、成本低、提取效率高、易富集到短片段的血浆游离DNA (cell-free DNA,cfDNA)提取试剂盒。方法 向健康人血浆中投入一定量的6种长度各异的非人源DNA,经5种试剂盒提取后,使用实时荧光定量PCR (Real-time quantitative PCR,qPCR)定量,比较5
Viagen Biotech, Inc科学家开发的专利组分(正在申请专利)允许用DirectPCR® DNA Extraction System获得的DNA提取物与基因分型用的基因组PCR兼容。生物样本的粗提物与许多分子生物学级的试剂比如PCR都难以兼容,部分原因在于粗提物内含有抑制剂。DirectPCR®不仅介导生物样品的快速裂解,而且含有抑制剂,能够有效抑制PCR...
Recent advances in single cell technology have enabled dissection of cellular heterogeneity in great detail. However, analysis of single cell DNA sequencing data remains challenging due to bias and artifacts that arise during DNA extraction and whole-gen
Cell-free DNA (cfDNA) has been implicated as an important biomarker in cancer management. Thus, efficient techniques for cfDNA extraction are necessary for precision medicine. We developed a centrifugation-free cfDNA extraction microfluidic chip capable of extracting cfDNA from plasma samples through micr...
DNA extractionDNAzolcell suspensioncytogeneticLEUKEMIAtext>We developed a procedure for DNA extraction from small volumes of fixed cell suspensions previously prepared for conventional cytogenetic analysis. Good quality DNA was isolated with a fast and simple protocol using DNAzol reagent. This provided ...
Protocol Springer Protocols(2024) Neural Stem/Progenitor Cell (NSPC) Extraction and Culture Jemma Gasperoni,Sebastian Dworkin Neural stem–progenitor cells (NSPCs) are multipotent, self-renewing cells that generate radial glial cells (RGC). RGCs then give rise to neurons and glia during neural deve...
However, molecular events such as transcription factor (TF)-DNA binding and their dissociation are temporal stochastic processes. The steady-state activity of a CRE in a cell is determined by the probability that such stochastic events occur over time. Since probability is a continuous measure, ...
This assay was conducted following the manufacturer’s protocol, using IU-TAB-1 cell lines. The quantification of the purified DNA sample was conducted using the qRT-PCR method. Immunofluorescence colocalization assay The immunofluorescence colocalization assay was performed using IU-TAB-1 cells ...
DNA methylation is a key epigenetic property that drives gene regulatory programs in development and disease. Current single-cell methods that produce high quality methylomes are expensive and low throughput without the aid of extensive automation. We previously described a proof-of-principle technique ...
Contemporary single-cell lineage-tracing (scLT) methods use expressed, heritable DNA barcodes to combine cell lineage readout with single-cell transcriptomics. However, reliance on transcriptional profiling limits adaptation to other single-cell assays. With CellTag-multi, we present an approach that ...