In all cases, this protocol allows to obtain good electrophoretic separation of proteins. As the protocol is rapid, universal, and compatible with silver staining, it could be used for routine protein extraction from recalcitrant plant tissues for proteomic analysis....
RNA extraction and quantitative RT-PCR analysis Total RNA was extracted from various tissues using TRIzolTM Reagent (Invitrogen). First-strand cDNA synthesis was carried out using 5× All-In-One RT MasterMix (Applied Biological Materials Inc.) according to the manufacturer’s instructions. The qPCR...
Genomic DNA was extracted from the leaves of each petunia cultivar using the DNeasy plant Maxi kit (QIAGEN, Venlo, Netherlands), following the manufacturer’s instructions.EcoRI andXbaI were used to digest 10 μg ofP. hybridagenomic DNA, which was then separated on a 0.9% agarose gel and...
FhSPL9, andFhSPL11) brought an enhanced expression of endogenousFLOWERING LOCUS Tleading to the significantly precocious flowering in transgenic Arabidopsis lines. Future study of the genes in the citrus plant itself is expected to conclude the assessments made in the current study....
Native protein extraction Unless otherwise stated, samples were quick-frozen and ground to a fine powder in liquid nitrogen using a chilled mortar and pestle. Powder was thawed to 4°C, and resuspended in approximately an equal volume of the specified lysis buffer plus protease and phosphatase inh...
cava, plants were divided into different parts: tubers, stems, leaves, flowers, and fruits. 2.2. Protein extraction The protein extract was prepared from tubers by mechanical homogenization (mortar and pestle) in liquid nitrogen. The resulting powder was dissolved in 0.1 M Tris-Cl buffer, pH ...
Phosphorylation of Rubisco activase is also very important for plant growth and development. Rubisco activase can promote the release of these analogs from the catalytic sites and maintain the enzyme in a catalytically active form. In the green alga Chlamydomonas reinhardtii, phosphorylation of Ser53 ...
Plant regeneration from leaf-derived protoplasts within the Daucus genus: effect of different conditions in alginate embedding and phytosulfokine application. Plant Cell, Tissue Organ Cult (PCTOC). 2014;117(2):241–52. Article CAS Google Scholar Nørholm MH. A mutant Pfu DNA polymerase ...
Additionally, selected plant species produce a minor fraction of tan protein at a higher coagulation temperature of ~82 C or show amorphous coagulation [42]. 3.3. Extraction and Concentration Crude isolation of green leaf proteins is achieved by pulping the leaves, pressing the pulp to obtain ...
Witzel, K.; Shahzad, M.; Matros, A.; Mock, H.P.; Mühling, K.H. Comparative evaluation of extraction methods for apoplastic proteins from maize leaves.Plant Methods2011,7, 48. [Google Scholar] [CrossRef] [PubMed] [Green Version] ...