Med23flox/flox;Lck-Cre mice have normal thymic populations, but MED23 null T-cells poorly populate the peripheral lymphoid tissues.Lawryn, H. KasperTomofusa, FukuyamaPaul, K. Brindle
in the thymuses of Med23flox/flox;Lck-Cre mice were essentially normal, MED23 null T-cells failed to efficiently populate the peripheral lymphoid organs... LH Kasper,T Fukuyama,PK Brindle - 《Plos One》 被引量: 3发表: 2014年 T-cells null for the MED23 subunit of mediator express decrea...
Conditional gene targeting using the bacteriophage-derived Cre recombinase is widely applied for functional gene studies in mice. Mice transgenic for Cre under the control of the lck gene promoter are used to study the role of loxP-targeted genes in T cell development and function. In this articl...
( conditional knock out mic ,cKO mice) 以及 L ck— Cre 转基 因小 鼠 ] ,借助 Cre—LoxP 位点 特异 性重组 酶 系统 ,构建 Perp 基 因在 免疫 系统 中特 异性敲除 的工 具小 鼠 ,并 以此 为基础进 一步 在整体动物水平 研究Perp 基 因在 T 细胞 发育 以及在 自身 免疫 疾病 发病 中...
因此 ,笔者从美国 Jackson实验 室 引进 了 Perp基 因条 件敲除鼠 (conditionalknockoutmic,cKO mice)以及 Lck— Cre转基因小 鼠 ],借助 Cre—LoxP位点特异性重组 酶系统,构建 Perp基 因在免疫系统 中特异性敲除 的工具小鼠,并以此为基础进一步在整体动物水平 表 2 P口rp 序列的PCR反应条件 研究 Perp基因在...
【关键词】 Perp;Lck-Cre;Cre-LoxP系统【中图分类号】 R392.5 【文献标志码】 AReproductionandGenotyping oftheLck-Cre×Perpflox/flox ConditionalKnock-OutMice LENG Xiao1,ZHOU Yan2,TANG Hai-ming1,ZHANG Xiang1,LIU Jia1,YANG Pei1,WANG Yan-tang1* (1.Department ofImmunology,School of Basic Medical...
Pten flox/flox Lck-Cre mice developed thymic lymphomas, but before the onset of tumors, they showed normal thymic cellularity. To reveal a regulatory ... TJ Hagenbeek,M Naspetti,F Malergue,... - 《Journal of Experimental Medicine》
Taken together, the data presented in this study shed new light on gamma delta T cell development and stimulate a reanalysis of data generated using the pLckCre transgenic mice.Fiala, Gina J.Univ FreiburgSchaffer, Anna-MariaLeibniz Res Inst Environm MedMerches, KatjaMorath, AnnaSwann, JeremyHerr...