自噬(Autophagy)是自噬溶酶体降解大部分细胞浆内容物的一种分解代谢过程。自噬通常会在营养缺乏的情况下被激活,同时又与许多生理病理过程相关,包括生长、分化、神经退行性疾病、感染和肿瘤等。 LC3(light chain 3)是目前公认的自噬分子标记物(autophagy marker)。 哺乳动物中有三种同工型LC3蛋白(LC3A、LC3B和LC3C),...
Sheep primary as- trocytes under starvation conditions express higher a- mount of LC3 II autophagy marker than neurons[J]. Arch Ital Biol,2014,152( 1) : 47 - 56.Mura E, Lepore G, Zedda M, et al. Sheep primary astrocytes under starvation conditions express higher amount of LC3 II auto...
自噬(autophagy)是在真核细胞中,过量或异常的细胞器被双膜泡隔离并交付给溶酶体/液泡后被降解,回收利用细胞性物质及清除受损细胞器或有害的蛋白质的过程。自噬的过程包括隔离膜(isolation membrane)或自噬前体(pre-autophagosome)的形成及延伸、自噬体(autophagosome)的形成、自噬体和溶酶体融合后形成自噬溶酶体(autolyso...
自噬(autophagy)是在真核细胞中,过量或异常的细胞器被双膜泡隔离并交付给溶酶体/液泡后被降解,回收利用细胞性物质及清除受损细胞器或有害的蛋白质的过程。 自噬的过程包括隔离膜(isolation membrane)或自噬前体(pre-autophagosome)的形成及延伸、自噬体(autophagosome)的形...
During autophagy LC3-I is subsequently conjugated to PE lipid as lipidated form: LC3-II. The lipidated form migrates faster on SDS-PAGE gel. The increased the level of LC3-II has been widely used as autophagy marker. This antibody recognizes both LC3-I (18-20 kDa) and LC3-II (14-16 ...
Drake KR, Kang M, Kenworthy AK. 2010. Nucleocytoplasmic distribution and dynamics of the autophagosome marker EGFP-LC3. PLoS One. 5(3):e9806 Guo GF, Jiang WQ, Zhang B et al. 2011. Autophagy-related proteins Beclin-1 and LC3 predict cetuximab efficacy in advanced colorectal cancer. World J...
栾智华,等 多种细胞自噬调节剂对自噬标记物LC3II及 p62表达的影响[J].中国药科大学学报,2018,49 (3):341-347 Citethisarticleas:WEIYanming,RENJinhong,LUANZhihua,etal Effectsofvariousautophagymodulatorsontheexpressionofautophagic markersLC3IIandp62[J].JChinaPharmUniv,2018,49(3):341-347 Effectsofvarious...
During autophagy LC3-I is subsequently conjugated to PE lipid as lipidated form: LC3-II. The lipidated form migrates faster on SDS-PAGE gel. The increased the level of LC3-II has been widely used as autophagy marker. This antibody recognizes both LC3-I (18-20 kDa) and LC3-II (14-16 ...
The autophagosome-associated protein LC3-II is commonly used as a marker of autophagic activity within cells, but its levels are affected by both formation and degradation of autophagosomes. This can make the significance of altered LC3-II levels ambiguous. Here we describe the method of Bafilomyci...
Autophagy marker Light Chain 3 (LC3) was originally identified as a subunit of microtubule-associated proteins 1A and 1B (termed MAP1LC3) (4) and subsequently found to contain similarity to the yeast protein Apg8/Aut7/Cvt5 critical for autophagy (5). Three human LC3 isoforms (LC3A, LC3B...