In this review, we discuss the current knowledge and function(s) of the LC3/GABARAP family proteins. We focus on processing of the individual family proteins and their role in autophagy initiation, cargo recognition, vesicle closure, and trafficking, a complex and tightly regulated ...
, it has been documented that all mammalian Atg8 homologues play a role in autophagosome biogenesis. Furthermore, because of unique features in the distribution of their molecular surface charges, it has been suggested that LC3 and GABARAP recognize distinct sets of cargoes for selective autophagy....
Both LC3 & GABARAP Are Essential for Autophagosome Formation. Autophagy is the main process for degrading damaged organelles and misfolded proteins. It plays a critical role in cell homeostasis, differentiation, survival during nutrient deprivation, and
英文摘要 Pathological cartilage calcification plays an important role in osteoarthritis progression but in which the origin of calcified extracellular vesicles (EVs) and their effects remain unknown. Here, we demonstrate that pathologic...
[Key words]Colorectal cancer; Autophagy; Tumor-associated macrophage; LC3; Tumor microenvironment 肿瘤的发生、发展与其所在的微环境密切相关,肿瘤免疫微环境的改变可通过调控细胞因子释放、改变肿瘤血管生成等多种途径影响肿瘤的发生、...
autophagyexpressed protein ligationLC3lipidated proteinsmembrane fusionprotein synthesisThe cover picture shows the synthesis of lipidated LC3 protein by using a combination of lipidated peptide synthesis and expressed protein ligation and investigation of the role of lipidated LC3 protein in membrane tethering...
Autophagyisthemajorproteindegradationsystemresponsiblefortheturnoverofbulkycellularconstituents(1).Asapivotalcellularhouse-keepingsystem,autophagycontributestomaintainintercellularhomeostasis (2).Inaddition,autophagyhasbeenfoundtoplaysignificantrolesinantigenpresentation,bacterialandviralinfection,andcelldeath(3–6).Dysfunc...
Microtubule-associated protein 1A/1B-light chain 3 (LC3) is a soluble protein with a molecular mass of ∼17 kDa that is distributed ubiquitously in mammalian tissues and cultured cells. During autophagy, autophagosomes engulf cytoplasmic components,
The most widely studied autophagy receptor shown to play an important role in autophagy is p62/SQSTM-1 (sequestosome-1)4,5. As these molecules are themselves autophagy substrates, their levels often increase when autophagy is perturbed5,6. Autophagosome numbers are widely assessed by quantifying ...
An important question regarding nuclear LC3 is whether and how it participates in autophagy. In this punctum, we discuss our recent findings about the essential role for nuclear LC3 in starvation-induced autophagy. During nutrient-rich conditions, LC3 is distributed in an acetylated form in both ...