The expression levels of autophagy markers were constant throughout the experiment in normal growth conditions. ConclusionsThis data showed that autophagy regulation is a dynamic process that coincides with changed and time dependent expression levels of LC3 and p62 in response to MG-132, Resvega and...
LC3 Western blot troubleshooting Significance of LC3-I/LC3-II ratio How to interpret LC3 immunoblotting Chloroquine-based positive controls Autophagy-inducing peptides ICC/IF and IHC staining of LC3 Anti-LC3B and anti-p62/SQSTM1 Analysis of autophagy flux markers Autophagy inhibitors and inducersWhat...
Samples were analyzed for autophagic markers LC3B and p62 in the peripheral, middle and central regions of the placenta and in omental adipocytes, milky spots and vasculature. Results: As pre-pregnancy BMI increased, there was an increase in both placental and fetal weight as well as decreased ...
d Representative western blot of autophagy markers from D2.0 R cells plated on BME or BME plus COL1 with or without HCQ on days 1 and 5. e Total lung surface metastatic burden in CD1nu/nu mice injected with D2.0R-GFP stably expressing shSCR (scrambled), shBECN1 (shRNA 1 and 3) ...
This may require new and more advanced intracellular markers, which can only be expected from further advances in this area of research. It is thought that overcoming these limitations will contribute to the improvement of strategies for the use of autophagy-targeting NPs in the field of tumor ...
Past studies have shown that autophagy activity in adipose tissue was significantly elevated in obese humans, and that the level of autophagy markers was significantly correlated with degree of obesity[7]. Kosacka et al., found that levels of the autophagy protein LC3 were significantly increased ...
The successful isolation of lysosomes without contaminating other intracellular organelles was demonstrated by Western blot with lysosome markers LAMP1 and LAMP2, Golgi marker GM130, mitochondria marker ATP5A or VDAC, and endosome marker EEA1. Remarkably, while Aurora B knockdown potentiated lysosome ...
induced cell death through apoptosis and autophagy stimulations. These observations were further confirmed in SKBR3 cells after Ag-NPs–EPS exposure in which the induction of autophagic markers was detected by fluorescence microscopy and western blot indicating a prominent mechanism of autophagic cell dea...
The contribution of the non-charge-selective, large-size leak pathway to autophagy-mediated changes in the TJ barrier appears to be minimal because autophagy induction did not affect the flux of paracellular markers having a molecular radius of >4 Å. The leak pathway allows the flux of ...
We further investigated these autophagic markers after HO-1 induction by CoPPIX. The results supported the upregulation of autophagy pathway. Similarly, we found significantly increased expression level of LC3B-II and p62 in CoPPIX 100 group compared to untreated cells. To confirm our Western blot...