However, the marker for transcriptional active chromatin, histone 2B Lys120 mono-ubiquitination was found reduced in USP36-silenced cells undergoing mitophagy. We observed a reduction of the mRNA and protein levels of Beclin-1 and its associated autophagy-related key regulator ATG14L in USP36 ...
Labeling lysosomes with a general endolysosomal marker such as LAMP1 (L1) allows confirmation that the fluorescent puncta are indeed lysosomes and allows us to determine the fraction of lysosomes engaged in CMA, because only lysosomes bearing Hsc70 in their lumen are competent for CMA38. Loss of...
Beta-tubulin III, a marker of differentiated neurons, increased ~tenfold by day 7 of differentiation (Fig. S4). PGC-1α B1E2 was barely detected in NSCs, increased ~12-fold by day 3 of differentiation, and persisted at an elevated level for the remainder of the differentiation process (...
ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells. 实验方案 Product Specific Protocols WB protocol for Alpha smooth muscle actin specific antibody 80008-1-RR Download protocol IHC protocol for Alpha smooth muscle actin specific ...
Cells that eliminate mitochondria through parkin RBR E3 ubiquitin protein ligase (PRKN)-mediated mitophagy are less sensitive to ferroptosis triggered by cystine starvation or erastin, but are more sensitive to ferroptosis induced by GPX4 inhibitors [26, 88, 89]. Increasing evidence indicates that ...
As Rho5 has been reported to trigger mitophagy (Schmitz et al., 2015), we also tested the deletion strains lacking either of the four MOMPs for this phenotype, using degradation of OM45-GFP as a readout. As evident from Fig. 2 the wild-type showed the expected reduction of the OM45...
High expression level of HOXA2, a marker for hindbrain progenitors, was additionally found during that time point in these cells. c, d Ventralization of cells was shown by positive IF staining of NKX2.2, NKX6.1, GATA2 and negative staining of PAX3/7, when treated with 1000 ng/ml SHH ...
(Figure1). During hypoxia-mediated nonselective macroautophagy, hypoxia-inducible factor-1α(HIF-1α) upregulates the expression of mitophagy marker BNIP3 (BCL2/adenovirus E1B 19 kDa-interacting protein 3) [22]. A detailed overview of the nonselective or canonical macroautophagy is narrated ...
Twenty-one GO terms related to purine nucleotide biosynthetic processes, mitophagy, regulations of mRNA processing and macromolecular glycosylation were statistically significant exclusively in the Female_Module16. Also, we found significant en- richment for the KEGG pathway (ko:04141- Protein pro- ces...
With the use of mito-Rosella, AUTAC4 was demonstrated to induce mitophagy in Detroit 532 cells ∼24–72 h after treatment (Figure S5K). Recruitment of an autophagy marker (LC3B) to the mitochondria was also observed (Figure 5J). Accelerated turnover of mitochondria in DS-derived fibroblasts...