High-throughput proteomics platforms measuring thousands of proteins in plasma combined with genomic and phenotypic information have the power to bridge the gap between the genome and diseases. Here we performed association studies of Olink Explore 3072
In HUNT 3, non-fasting plasma samples were collected in EDTA tubes at random times of day. The samples were centrifuged, plasma aspirated and frozen at −80 °C within 24 h of blood draw. Never-thawed samples were shipped to SomaLogic, Boulder, CO for proteomic profiling using the e...
METHODS. We characterized the plasma proteome of PLWH and PWoH using an aptamer-based platform and assessed univariable associations with incident heart failure. Druggable proteins were identified using the Therapeutic Target Database [2]. We built a protein interaction network querying significant ...
Both Olink and SomaScan use dilution groups for their assays based on the protein abundance in plasma, with the most abundant proteins belonging to the highest dilution groups (Supplementary Table7). For both platforms, the correlation between protein levels was lowest in the lowest dilution group ...
Isobaric labeling-based mass spectrometry (ILMS) methods, such as tandem mass tagging (TMT) [1] and isobaric tags for relative and absolute quantitation (iTRAQ) [2,3], have been widely used for quantitative proteomic profiling, showing unique advantages in a broad range of biological studies [...
Pathophysiological evolutions in early-stage Alzheimer’s disease (AD) are not well understood. We used data of 2923 Olink plasma proteins from 51,296 non-demented middle-aged adults. During a follow-up of 15 years, 689 incident AD cases occurred. Cox-proportional hazard models were applied to...
Proteomic analysis of urine exosomes by multidimensional protein identification technology (MudPIT) Exosomes are membrane vesicles that are secreted by cells upon fusion of multivesicular bodies with the plasma membrane. Exosomal proteomics has emerged as... Z Wang,SJ Hill,JM Luther,... - 《Proteomics...
Heart-brain signaling in patent foramen ovale-related stroke: differential plasma proteomic expression patterns revealed with a 2-pass liquid chromatography-tandem mass spectrometry discovery workflow. J. Invest. Med. 2012, 60 (8), 1122−1130. (34) Qu, J.; Lesse, A. J.; Brauer, A. L.;...
4c). In branch secondary xylem (Additional file 8), all categories showed similar results to stem secondary xylem (Additional file 9), except for the protein transport through plasma membrane function. Catabolic process and response to stress (biological process), nucleotide and protein binding (...
The plasma proteomic changes that precede the onset of dementia could yield insights into disease biology and highlight new biomarkers and avenues for intervention. We quantified 4,877 plasma proteins in nondemented older adults in the Atherosclerosis Risk in Communities cohort and performed a proteome...