plasma aspirated and frozen at −80 °C within 24 h of blood draw. Never-thawed samples were shipped to SomaLogic, Boulder, CO for proteomic profiling using the equivalent assay (SOMAScan v4). Nine samples failed SOMALogic quality control and were excluded...
High-throughput proteomics platforms measuring thousands of proteins in plasma combined with genomic and phenotypic information have the power to bridge the gap between the genome and diseases. Here we performed association studies of Olink Explore 3072
Large-scale proteomic profiling reveals characteristic HIV-specific druggable protein signatures associated with incident heart failuredoi:10.1093/eurheartj/ehae666.3243BACKGROUND. Compared with the general population, persons with HIV suffer from an augmented risk of both systolic and diastolic heart ...
Both Olink and SomaScan use dilution groups for their assays based on the protein abundance in plasma, with the most abundant proteins belonging to the highest dilution groups (Supplementary Table7). For both platforms, the correlation between protein levels was lowest in the lowest dilution group ...
Isobaric labeling-based mass spectrometry (ILMS) has been widely used to quantify, on a proteome-wide scale, the relative protein abundance in different biological conditions. However, large-scale ILMS data sets typically involve multiple runs of mass sp
Isobaric labeling-based mass spectrometry (ILMS) has been widely used to quantify, on a proteome-wide scale, the relative protein abundance in different biological conditions. However, large-scale ILMS data sets typically involve multiple runs of mass sp
We describe a largely unbiased method for rapid and large-scale proteome analysis by multidimensional liquid chromatography, tandem mass spectrometry, and database searching by the SEQUEST algorithm, named multidimensional protein identification technology (MudPIT). MudPIT was applied to the proteome of the...
Catabolic process and response to stress (biological process), nucleotide and protein binding (molecular function), plastid and plasma membrane (cellular component) are the main categories for both tissues (Additional file 9). Further, three heat-shock proteins (TgHsp1, TgHsp2 and TgHsp3), one ...
Walker, K.A., Chen, J., Zhang, J.et al.Large-scale plasma proteomic analysis identifies proteins and pathways associated with dementia risk.Nat Aging1, 473–489 (2021). https://doi.org/10.1038/s43587-021-00064-0 Download citation ...
when tested individually. Future large-scale DIA-MS experiments on blood plasma from animals with sepsis and human sepsis patients using the tissue atlas spectral library will more accurately enable the correlation between groups of biomarkers and disease progression, disease severity and disease outcome...