By integrating thermal stability of proteoforms, transcriptomics and drug sensitivity profiling data across cell lines, we demonstrate that it is possible to identify biomarkers for cellular processes and drug response. Thus, we think that deep TPP for proteoform detection is a broadly applicable and ...
Thermal proteome profiling (TPP) methodology has the potential to characterize proteome-wide stability highlighting key proteins during T. cruzi infection and life stage transition from the invertebrate to the mammalian host. In the present work, T. cruzi epimastigotes and trypomastigotes cell ...
METAL-TPP profiling with another chelator, TPEN, uncovered additional MBPs in proteomes. Collectively, this study developed a robust tool for proteomic discovery of MBPs and provides a rich resource for functional studies of metals in cell biology. This is a preview of subscription content, access...
METAL-TPP profiling with another chelator, TPEN, uncovered additional MBPs in proteomes. Collectively, this study developed a robust tool for proteomic discovery of MBPs and provides a rich resource for functional studies of metals in cell biology. This is a preview of subscription content, log ...
Thermal proteome profiling in cell extracts Na-ATP, Mg-ATP, and Na-GTP stocks (300 mM) were prepared in PBS containing 1.5 mM MgCl2 and the pH was adjusted to 7 using NaOH. For TPP-temperature range (TPP-TR) experiments11, two parts of the lysates (both crude and gel filtered),...
Thermal proteome profiling (TPP) analysis After normalization of the raw TPP-TR data, the TPP package (Franken et al., 2015) was used to identify melting points. After normal- ization of the 2D-TPP dataset, fold changes were calculated against the respective 37C and the G1/S data ...
Mass spectrometry based CEllular Thermal Shift Assay or Thermal Proteome Profiling (TPP) is a recent method that can assess system-wide protein binding to small molecules, metabolites, or nucleic acids by monitoring changes in protein thermal stability19,20. Similar techniques such as stability of pr...
An implementation of the above described CS-TPP analysis procedure can be found athttps://github.com/mathiaskalxdorf/RTSAor athttps://doi.org/10.5281/zenodo.4274152. References Download references Acknowledgements We thank J. Stuhlfauth, N. Garcia-Altrieth, K. Beß and B. Dlugosch for the...
Fig. 5: DLPTP recovers annotated GTP and ATP interactors from a 2D-TPP experiment profiling GTP–protein interactions. aVolcano plot for the analysis of the GTP gel-filtered lysate dataset.bUpset plot of set intersections of annotated nucleotide-binding proteins, stabilized hits found with the thr...
We have used a mass spectrometry-based proteomic approach to compile an atlas of the thermal stability of 48,000 proteins across 13 species ranging from archaea to humans and covering melting temperatures of 30–90 °C. Protein sequence, composition a