这些发现提示YAP在KCs中调节NAFLD中细胞因子的产生和肝脏炎症的重要作用。与HFD喂养的WT小鼠相比,来自HFD喂养的YAPϕKO小鼠的KCM1标记分子(CD80和CD86)明显减少,而M2标记分子(Mgl2和Retlna)明显增多。 图2:KCs/巨噬细胞中YAP的缺失可减...
regulatePPARγexpressionmeanwhile.Cellculturesupernatantswerecollectedto prepareconditionedmedium(CM).M1/M2phenotypemarkersweredetectedby Real-timePCR.HepatocytesweretreatedwithdifferentfattyacidsordifferentCM. Real-timePCRandWesternBlotwereusedtodetectNLRP3inflammasomerelated ...
在此,将小鼠和人单核、巨噬细胞M1、M2、Kupffer细胞、DC表型汇总表分享如下,大家可根据需要,选择鉴别能力强的指标: 表格来源:Cossarizza A, Chang HD, Radbruch A, et al. Guidelines for the use of flow cytometry and cell sorting in ...
Although macrophages contribute to cancer cell dissemination, immune evasion, and metastatic outgrowth, they have also been reported to coordinate tumor-specific immune responses. We therefore hypothesized that macrophage polarization could be modulated
93 INTERLEUKIN-10 RELEASED BY M2 KUPFFER CELLS PROMOTES M1 KUPFFER CELL APOPTOSIS: A NOVEL PROTECTIVE MECHANISM AGAINST ALCOHOLIC LIVER DISEASE - Journal of Hepatologydoi:10.1016/S0168-8278(12)60107-XJ. WanM. BenkdaneF. Teixeira-ClercV. Deveaux...
Modulation of PPAR-γ activity on lipid-induced macrophages M1/M2 shifting. Our in vitro cell models indicated that PPAR-γactivation was essential for lipid-induced M2 macrophage phenotype. To further ScIenTIfIc Reports | 7:44612 | DOI: 10.1038/srep44612 3 www.nature.com/scientific...
2.1.2Kupffer cell Kupffer cellsare macrophages that are specifically located in the liver sinusoids, and they participate in the front line of defense of the liver's immune system. Kupffer cells can be activated by lipopolysaccharide (LPS), complement system and other pathogen associated molecular ...
在抗肿瘤方面,活化的 M1 型巨噬细胞一方面发挥抗原提呈细胞(Antigen Presenting Cell, APC)的作用,将肿瘤抗原提呈给 T 淋巴细胞及 T 淋巴细胞分化的亚群,使得肿瘤细胞更高 效的被特异性清除[25],另一方面通过其吞噬功能将肿瘤细胞吞噬,吞噬细胞内的溶解酶与肿瘤细胞结合 后将肿瘤细胞杀死。活化的 M2 型巨噬细胞...
公司自成立以来便一直秉承“精品、专业、诚信、便捷"的宗旨和理念,不断的开拓进取,务实创新,收录与典藏了近千种各类细胞株/系,公司细胞主要来源ATCC,ECACC,ScienCell, DSMZ, RIKEN,JCRB 等,以及少数国内外科研机构建系。其中自主研发建系各类示踪细胞、耐药株细胞、基因敲除细胞等百余种,客户遍及全国各地的医院、...