Kupffer cellMulticolor flow cytometry and cell sorting are powerful immunologic tools for the study of hepatic m?, yet there is no consensus on the optimal method to prepare liver homogenates for these analyses. Using a combination of m? and endothelial cell reporter mice, flow cytometry, and ...
表格来源:Cossarizza A, Chang HD, Radbruch A, et al. Guidelines for the use of flow cytometry and cell sorting in immunological studies. Eur J Immunol. 2017 Oct;47(10):1584-1797. 人巨噬细胞M1和M2的区分,大多用CD11c和CD206...
(jandk). Flow cytometry data are representative of 2 independent experiments. BMDM, bone marrow derived macrophage; FCM, flow cytometry; iPo, intraportal; KC, Kupffer cell; scRNAseq, single cell RNA sequencing; Ctrl, control; IFN, interferon; MHC, major histocompatibility complex; APC, antigen...
Information complied from numerous sources, including published research articles and catalogues of companies that specialize in supplying fluorescent probes to immunologists and cell and molecular biologists. Many of the probes listed above are employed in flow cytometry. I have utilized the Handbook of ...
Quality Control 1) Flow cytometry utilized to analyze cell surface markers F4/80, CD11b, CD14, and TLR4; 2) Western blot analysis on p53 expression; 3) qRT-PCR used to analyze level of LPS induced cytokine production Disclaimer 1. The CoA for this product (provided upon request) verifies...
Lima Silva Santos with flow cytometry and cell sorting. We thank members of the Lyden laboratory for insightful discussions. We gratefully acknowledge support from the National Cancer Institute (CA224175 (D.L.), CA210240 (D.L.), CA232093 (D.L.), CA207983 (D.L.), CA169538 (D.L.)...
fungi, and mycoplasma. Cells are tested for expression of markers using antibodies, CD11b by flow cytometry. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by AcceGen. Repeated freezing and thawing of cells is not recommended. ...
采用流式细胞术(flowcytometry,FCM)检测出血组与非出血组中KC表达的 ROS(Reactive oxygenspecies,活性氧)水平。KC采用连接有荧光素的CDllb抗 体进行免疫标记反应。ROS的生成可利用对氧化敏感的荧光染料DCFH.DA(终浓 度10岬)检测【8】。 取一80"C冰箱内的标本剪成lmm’左右的碎块并研磨 ...
we found that the cell-surface transmembrane protein ERMAP expressed on various cancer cells signaled to activate phagocytosis in Kupffer cells and to control of liver metastasis. ERMAP interacted with β-galactoside binding lectin galectin-9 expressed on the surface of Kupffer cells in a manner dep...
flow cytometry (FCM)to detect the expression of F4/80 antibody andthe ink uptake test to observe the phagocytic activity. Moreover,using FCM,we evaluated the expressions of moleculesassociated with antigen presentation,including major histocompatibility complex class Ⅱ (MHC Ⅱ),CD40,CD86 and CD68on...