首页/癌基因/KRAS/ AMPLIFICATION KRAS (Kirsten Rat Sarcoma Viral Oncogene Homolog)基因是GDP/GTP结合蛋白,比较重要的同家族基因还包括HRAS和NRAS。KRAS与GTP结合呈激活状态,与GDP结合呈关闭状态,KRAS可被生长因子或酪氨酸激酶(如EGFR)短暂活化,活化后的KRAS可以激活下游如控制细胞生成的PI3K-AKT-mTOR信号通路,以及控...
More recently, KRAS amplification was discussed as a new therapeutic target. Little is known about the (prognostic) relevance and (heterogenic) distribution of KRAS amplification in gastric adenocarcinomas, especially in Non-Asian patients. Methods: Amplification of the KRAS locus and corresponding ...
the functional role ofKRASWTamplification, which has recently been shown to be a secondary means of KRAS activation in cancer2, remains mostly unexplored. The largest part of the human genome is composed of non-coding genes, which are transcribed but ...
基因检测 A组:采用amplification refractory mutation system (ARMS)-PCR 检测了1239例患者,103例KRAS突变(8.3%) 在这个组里,发现相比于EGFR突变患者,KRAS突变患者的吸烟史比例和男性比例更高,年龄更大一些。 B组: 采用next-generation sequencing (NGS) 检测了3941例患者,368例KRAS突变(9.3%),其中1例为KRAS和NRAS...
AmplificationRetrovirusCAsE-PE cells are an arsenic-transformed, human prostate epithelial line containing oncogenic mutations in KRAS compared to immortalized, normal KRAS parent cells, RWPE-1. We previously reported increased copy number of mutated KRAS in CAsE-PE cells, suggesting gene amplification. ...
临床上,常用的分子学检测技术有 Sanger 测序和 PCR,但两种方法均有不同程度的敏感性局限。不过,目前敏感性更高的分子学技术(amplification refractory mutation system 突变阻滞扩增系统,ARMS)已经进入临床。 通常情况下,癌灶中不同组织学表型的肿瘤组织,其突变驱动基因(driver gene)也不同。同样,肺癌也具有瘤内异质...
这种“开”的信号导致细胞的生长和增生(proliferation)。因此RAS的过度表达(overexpression)与扩增(amplification)导致持续的细胞增殖,这是肿瘤发生的关键一步。细胞分裂由“正”与“负”信号的平衡来调控。当RAS转录增加时,过多的基因蛋白积蓄在细胞内;此时,细胞分裂的“正”信号则强于“负”信号。
Barzi A, Weipert CM, Espenschied CR, et al. ERBB2 (HER2) amplifications and co-occurring KRAS alterations in the circulating cell-free DNA of pancreatic ductal adenocarcinoma patients and response to HER2 inhibition. Front Oncol. 2024;14:1339302. Published 2024 Feb 8. doi:10.3389/fonc.2024.1...
①ARMS(amplification refractory mutation system)法:该方法是利用与荧光探针相连的特定引物(蝎形或环形)进行的即时定量PCR。通过将与探针部的突变部位结合的邻近碱基(第2个碱基)与错配的碱基替换,并与阻断延伸反应的“ARMS”技术结合,提高特异性,从而可以进行高灵敏度测定...
因此ras的过度表达（overexpression）与扩增（amplification）导致持续的细胞增殖，这是肿瘤发生的关键一步。细胞分裂由“正”与“负”信号的平衡来调控。当ras转录增加时，过多的基因蛋白积蓄在细胞内；此时，细胞分裂的“正”信号则强于“负”信号。 ras基因的点突变（pointmutation）通常导致ras从原癌基因（proto-...