临床上,常用的分子学检测技术有 Sanger 测序和 PCR,但两种方法均有不同程度的敏感性局限。不过,目前敏感性更高的分子学技术(amplification refractory mutation system 突变阻滞扩增系统,ARMS)已经进入临床。 通常情况下,癌灶中不同组织学表型的肿瘤组织,其突变驱动基因(driver gene)也不同。同样,肺癌也具有瘤内异质...
Wild-typeKRAS(KRASWT) amplification has been shown to be a secondary means of KRAS activation in cancer and associated with poor survival. Nevertheless, the precise role ofKRASWToverexpression in lung cancer progression is largely unexplored. Here, we identify and characterize a KRAS-responsive lncR...
KRAS Amplification in a 73-Year-Old Man with Adenocarcinoma of LungKRAS Amplification in a 73-Year-Old Man with Adenocarcinoma of Lung - Cancer Geneticsdoi:10.1016/j.cancergen.2014.06.014elsevierCancer Genetics
[2].Socinski MA, Pennell NA, Davies KD. MET Exon 14 Skipping Mutations in Non-Small-Cell Lung Cancer: An Overview of Biology, Clinical Outcomes, and Testing Considerations. JCO Precis Oncol. 2021;5:PO.20.00516. [3].Tong ...
临床上,常用的分子学检测技术有Sanger测序和PCR,但两种方法均有不同程度的敏感性局限。不过,目前敏感性更高的分子学技术(amplificationrefractorymutationsystem突变阻滞扩增系统,ARMS)已经进入临床。 通常情况下,癌灶中不同组织学表型的肿瘤组织,其突变驱动基因(drivergene)也不同。同样,肺癌也具有瘤内异质性,主要由腺...
[2].Socinski MA, Pennell NA, Davies KD. MET Exon 14 Skipping Mutations in Non-Small-Cell Lung Cancer: An Overview of Biology, Clinical Outcomes, and Testing Considerations. JCO Precis Oncol. 2021;5:PO.20.00516. [3].Tong JH, Yeung SF, Chan AW, et al. MET Amplification and Exon 14 ...
不过,目前敏感性更高的分子学技术(amplificationrefractorymutationsystem突变阻滞扩增系统,ARMS)已经进入临床。 通常情况下,癌灶中不同组织学表型的 组织,其突变驱动基因(drivergene)也不同。同样,肺癌也具有瘤内异质性,主要由腺鳞癌和小细胞癌组成。 确定肺癌中不同形态学表型组织的驱动基因,对肺癌患者的个体化治疗至...
KRAS and EGFR Amplifications Mediate Resistance to Rociletinib and Osimertinib in Acquired Afatinib-Resistant NSCLC Harboring Exon 19 Deletion/T790M in EGFR. Mol. Cancer Ther. 2019, 18, 112–126. [Google Scholar] [CrossRef] [Green Version] Ihle, N.T.; Byers, L.A.; Kim, E.S.; Saint...
Real-time PCR amplification was performed in the C1000 Touch Thermal Cycler (BioRad) using the PerfeCTa SYBR Green FastMix Reaction Mix (QuantaBio, #101414-276). Beta-2 microglobulin was used as an endogenous control and differences between samples were calculated using the 2-ΔCt (delta CT...
Clinical Cancer Research 361 Table 1 Summary of PCR primers, amplification products, restriction enzymes, genes and diagnostic restriction fragments for the K-ras and H-ras PCR Primers K-ras Codon product (6p)(’ Restriction enzyme Mutantspecific fragment (bp) Wild allele- specific fragment (bp...