we identified high level amplification of theKRASgene, as previously reported12, as well KRAS copy number gain (see methods) in both lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC), with consequent increase ofKRASmRNA (Fig.1aand Supplementary...
one of the mediators of many cellular signaling pathways, reciprocally cause diverse molecular alterations, such as dysregulation of lncRNA expression.KRASamplification has been shown to be a secondary means of KRAS activation, leading to its overexpression and neoplastic transformation. It was found tha...
typeKRASpromoter G4 (for convenience, theKRAS-G4 refers to Pu24m1 DNA afterward). Fig. 1:KRAS-G4 and its interaction with berberine and coptisine. aSchematic of the humanKRASgene promoter and the formation of aKRAS-G4 as well as its complex with small molecules (ligands). The G4-for...
Besides non-G12C KRAS mutations, amplifications of native KRAS are frequently found in pancreatic cancer. BI-3406 is a potent SOS1 inhibitor that is orally bioavailable and active in cells with KRAS mutations, but not on KRAS wild-type cells [21]. BAY-293 is a SOS1 inhibitor with high ...
RTK: receptor tyrosine kinase; Mut: mutation; Amp: amplification. Table 1. Ongoing clinical trials assessing combination therapies including covalent KRAS G12C inhibitors. An epithelial-to-mesenchymal cell transition (EMT) program contributes to the therapeutic resistance to various types of ...
also observed some patients with high-level focal amplifications of the KRAS (G12C) allele [94]. Xue et al. found the increased new KRAS (G12C) protein in KRAS (G12C)-mutant tumor cells and elucidate that EGFR can promote the conversion of KRAS from the GDP state to the GTP-bound...
Analytical sensitivity* of about 1% demonstrated with genomic DNA and model FFPE cell line dilutions Includes internal endogenous control (EC) to assess sample DNA quality/purity External positive and negative plate controls provided independently to assess the validity of the amplification, hybridization...
DNA and RNA were set with different temperature programs in the amplification instrument for PCR amplification; (7) Quantitative analysis and signal statistics were carried out on the amplification products after the amplification was completed, and the detection results were obtained. Statistical analysi...
Microfluidic processing of the GeneSlice along with allele-specific amplification and real-time detection are conducted in a slightly modified, commercially available PCR thermocycler. Intra-chip standard deviation of Cq values on the GeneSlices is negligible (GeneSlice 1: Cq,std.dev. = 0.13; Gene...
Analytical sensitivity* of about 1% demonstrated with genomic DNA and model FFPE cell line dilutions Includes internal endogenous control (EC) to assess sample DNA quality/purity External positive and negative plate controls provided independently to assess the validity of the amplification, hybridization...