胞对K562/AO2细胞杀伤活性降低与HLAI类分子无关;MICA/B在多药 耐药肿瘤细胞的低表达导致耐药肿瘤细胞对NK细胞杀伤敏感性下降 关键字:自然杀伤细胞;MHCI类链相关分子A/B;K562细胞 ExpressionofHLAClassIMoleculesandMHCClassI ChainrelatedMoleculesA/BinK562andK562/AO2CellLines ...
K562 is the most widely used cell-based aAPC. This cell line lacks surface expression of human leukocyte antigen (HLA) class I and II molecules. Unlike magnetic beads, K562 can be readily manipulated to express additional co-stimulatory molecules by viral transduction [60,61]. We initially engi...
目的:与经典HLA I类分子相比,HLA-G分子的组织分布有限.甲基化导致的基因沉默现象是表观遗传学领域研究的热点之一,本文研究K562细胞系HLA-G基因启动子区甲基化状态及其与基因表达的关系.方法:应用亚硫酸氢钠脱氨基-DNA测序(bisulphite sequencing)的方法检测经5-氮胞苷(5-aza-2'-deoxy- cytidine)处理前后的K562细胞...
靶细胞表达HLAG1分子可明显抑制NK细胞的杀伤效应: :关键词:HLAG1;NK细胞;转染 [中图分类号] R39212 [文献标识码] A ExpressionofHLA--G1antigenonK562cellinhibitscytotoxicityme-- diatedbyNKfromPBMC HANJunyan.HUANGYa—fei,WUXiongwen,GONGFei—li ...
Both phenomena can prevent the detection of low frequency T cell responses in PBMC. In search of alternative APC for ELISPOT assays, the human chronic myelogenous leukemia cell line K562 that per se does not express HLA class I and class II molecules on the cell surface was transfected with ...
K562和K562/AO2细胞HLA I 类分子与M I CA /B 的 表达及其对NK 细胞杀伤活性的影响 梅家转,牛新清,郭坤元,周健,魏红梅 南方医科大学珠江医院血液科,广州510282 摘要 本研究探讨K 562亲本细胞株及多药耐药细胞株K 562/AO2细胞表面HLA I 类分子和MH C I 类链相关分子(M ICA /B)的表达及其对NK 细胞杀伤...
结果PMA联合CI组细胞形态发生明显变化,表面出现许多细小的突起,细胞表面CD83、CD86、CD80、CD40、HLA DR和CD1a表达上调,并且可以刺激淋巴细胞的增殖反应。结论PMA联合CI可以有效地使K562细胞向DC分化。 【关键词】K562细胞树突细胞十四酰佛波乙酯钙通道离子载体
Problem: Due to high abundance of decidual CD56+ NK cells in close contact with extravillous trophoblast cells, influence of HLA-G molecule on FasL-mediated cytotoxicity was investigated. Methods of study: Decidual mononuclear cells were isolated by density gradient and decidual lymphocytes were collec...
A human T cell culture (HLA A2; w23 B40; w44 Cw4) was established by repetitive stimulation with an Epstein Barr virus-transformed B cell line JY in a seru... H Spits,H Ijssel,C Terhorst,... - 《Journal of Immunology》 被引量: 233发表: 1982年 Establishment, characterization, and ...
摘要本研究目的是建立稳定表达HLA-A * 1101分子的K562细胞株,为研究慢性髓系白血病(CML)HLA-I限 制性抗原特异T细胞的细胞毒作用提供靶细胞。利用RT-PCR从CML患者外周血单个细胞中扩增HLA-A * 1101 基因全长序列;利用重组PCR将2A肽连接子(D-V-E-X-N-P-G-P)基因连接到HLA-A ...