The induction of class II antigen (Ag) by interferon-γ (IFN-γ) in variants of the K562 cell line has been examined in this study. Following incubation of K562A cells with IFN-γ, surface expression of HLA-DR molecules was demonstrated by indirect immunofluorescence, confirmed by immunoblotti...
Stanley R. Ress , Jeanne Rousseau , Bharati Ratanjee , Karen Eidne , Robert P. Millar , Mustapha Keraan . (1991) HLA class II induction by interferon-γ in K562 variant cell line: inhibition by serum lipid. Human Immunology 31 , 57-66 Online publication date: 1-May-1991. /...
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer (50mM Tris(pH 7.4),150mM NaCl,1% Triton X-100, 1% deoxycholate, 0.1% SDS) with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay. ...
关键词: Animals Humans Megakaryocytes K562 Cells Rotavirus Phorbol 12,13-Dibutyrate Receptors, Collagen Cell Differentiation Integrins DOI: 10.1007/s007050170069 被引量: 3 年份: 2001 收藏 引用 批量引用 报错 分享 全部来源 求助全文 全文购买 Springer 国家科技图书文献中心 (权威机构) 掌桥科研 万方医学...
The preservation place is China Center for Type Culture Collection. The molecular weight of the HLA-G1 antigen produced by the cell strain is 39 kDa. The HLA-G1 antigen comprises heavy chains, a light chain and antigen peptides. The heavy chains comprise Alpha 1, Alpha 2 and Alpha 3 ...
Somatic Cell Genet. 1982 Nov; 8 (6):775–789.Ziegler A, Uchanska-Ziegler B, Zeithen l: HLA expression at a single cell level on a K562 and B cell hybrid: An analysis with monoclonal antibodies using bacterial binding assays. Somat Cell Genet 1982, 8:755-789...
HLA antigen expression at the single cell level on a K562 x B cell hybrid: an analysis with monoclonal antibodies using bacterial binding assays. Somatic Cell Mol Genet 1982; 8:775-89.Ziegler A, Uchańska-Ziegler B, Zeuthen J, Wernet P (1982) HLA antigen expression at the single cell ...
In Vitro Expansion of Antigen-Specific Cytotoxic T Lymphocytes by HLA-A*0201 Transfected K562 for Monitoring of T-cell Responsesdendritic cellsimmunotherapyserum-free mediumanti-interleukin-10Monocyte-derived dendritic cells (MoDCs) in clinical use for cancer immunotherapy are ideally ...
Cells HeLa, K562, and HEK293T cell lines were purchased from the American Type Culture Collection, Manassas, VA, USA. HeLa and HEK293T were cultured in Dulbecco's modified Eagle's medium supplemented with 10% heat-inactivated foetal calf serum and 2 mM L-glutamine (Lonza Cologne AG, ...