The human leukemia cell line K562 represents an attractive platform for creating artificial antigen-presenting cells (aAPC). It is readily expandable, does not express human leukocyte antigen (HLA) class I and II and can be stably transduced with various genes. Methods. In order to generate ...
Next days, CAR-T cells and irradiated K562-aAPC #1D2 were co-cultured (ratio of 1:2) [19]. Flowcytometry to detect antigen-presenting cells: Flowcytometry analysis was performed on BD FACS Canto II (Germany), following the guidance of manufacture. Firstly, parental T cells were transfected...
K562cellsthatexpressesthereceptorOfIgGFc.Whichlaysasolidfoundationforconstructing theartificiaIAPCsinfuture. KEY WORDS:CD32;K562cells;expression lgGFc段受体一直是连接体液免疫和细胞免 疫的纽带。通过该受体,lgG可介导白细胞的很 多功能,包括ADCC,调理吞噬作用,吞噬细胞中 ...
结论:使用不同的起始细胞和aAPC可以获得GMP级NK细胞供临床使用。 高效摇瓶 In this report, we optimized a protocol to obtain NKAE cells by using four different culture growth media (RPMI, SCGM, TexMACs and NKMACs), two different NK cell sources: PBMC or CD45RA+cells and two distinct irradiat...