1.8万 19 37:12 App 详细的分子克隆实验操作过程 3416 -- 23:47 App 如何操作才能抽提出优质的质粒 Plasmid DNA Isolation 4.4万 56 25:25 App 质粒图谱怎么看 7477 2 1:03:43 App 分子克隆-质粒构建基础知识--唯问生物Justscience 5140 -- 5:00 App 大肠杆菌质粒转化实验 4975 1 21:23 App ...
KG1D, could remove As efficiently irrespective of changes in the media pH. In contrast, the efficiency of metal removal by PF14 was largely pH-dependent. The cell mass of both the isolates was also found to favourably adsorb As(III). Whole genome sequence analysis...
2.5. DNA Extraction and Manipulation DNA from bacteriophage lysates was isolated using Phage DNA Isolation Kit (Norgen Biotek, Ontario, Canada) according to the manufacturer’s instructions. Extraction of bacterial DNA and of DNA from a smaller volume of purified and concentrated phage culture (2 ×...
The mechanism of DNA transfer in the mating system of an archaebacterium. Science 245, 1387–1389 (1989). Article ADS CAS PubMed Google Scholar Imachi, H. et al. Cultivation of methanogenic community from subseafloor sediments using a continuous-flow bioreactor. ISME J. 5, 1913–1925 (...
14.The method of claim 1, further comprising the step of determining RNA with DNA microarray technology, wherein isolated RNA is amplified or cloned, added to an array of immobilized polynucleotides, hybridization to immobilized polynucleotides is measured, and the presence and / or identity of ...
LD003. Experiment performed in duplicate. Full size image P. norimbergensis LD001 appeared to decolourize a broad range of dyes in the liquid assays, among which the triarylmethane dye Malachite green (MG) and the indigoid dye Indigo Carmine (IC). However, the abiotic controls of these ...
2.8. Antiviral effects of mAbs in mice Two sets of in vivo experiments were performed by intranasally inoculating mice with the QHmusX virus (2.3 × 103 TCID50/mouse) (Iwata-Yoshikawa et al., 2022). In the first experiment, 2.5–30 mg/kg mAbs of EV053273, EV053286, cocktail (EV0532...
The feeding experiment was conducted under a 12 h:12 h light-dark cycle, the water temperature was 25 °C, and 80% of the water in the tanks was replaced every 2 days to maintain the quality of the water. 2.5. Growth performance and survival At the end of the experiment,...
After 12 days of incubation at 28°C, fermentation filtrates were extracted with three half-volumes of ethyl acetate (EtOAC), and the crude extracts were separated and dried using rotary evaporator at 40°C and 240 mbar.27 Phylogenetic tree full of 16S rDNA gene sequencing DNA extraction...
in parallel are used as controls. Each imaging experiment was independently performed at least three times with similar results, and representative images are shown (b–d).eViral titers of infected Huh7 cells at different time points post-infection.n = 3 biologically independent experiments ...