Alessio Scarafoni,Marcello D.An approach to the critical assessment of the experimental conditions in practical molecular biology:isolation of plant DNA.Biochemistry and Molecular Biology Education. 2001Aleasio S,Marcello D.An approach to the critical assessment of the experimental conditions in practical...
Current protocols for inclusion body isolation from Escherichia coli usually offer between 95 to 99% of protein recovery, what in practical terms, might imply extensive bacterial cell contamination, not compatible with the use of inclusion bodies in biological interfaces. Results Using an appropriate ...
<sec>Background<italic>Shigella</italic> is a class of pathogenic bacteria that can cause diarrhea in humans and animals. As the problem of drug resistance is becoming more and more serious due to the abuse of antibiotics, alternative a
According to the results from PhageTerm, a fixed position was recognized on the DNA of R16F and 353-bp direct terminal repeats (DTR) were observed, suggesting that the DNA termini of R16F belong to the DTR class. A total of 260 ORFs were identified in the genome of R16F, of which ...
The emergence of pyrethroid resistance is a major concern for vector control strategies as it threatens the efficiency of the insecti- cide treated nets, all of which contain this insecticide class, that have proven so successful ...
A change in the configuration of DNA occurred due to the binding of the aptamers to the cell targets, increasing the distance between the dye and the GO and subsequently recovering the fluorescence signal. As an alternative, a "turn-on" detection method using a microarray modified with rGO ...
In the scenario of rising antibiotic resistance among serious pathogens, the re-emergence of phage therapy has brought hope and a paradigm shift in the development of a new class of antibacterial. Harnessing the lytic activity of phages against specific bacteria has shown to be an effective ...
Many studies correlate changes in human gut microbiome with the onset of various diseases, mostly by 16S rRNA gene sequencing. Setting up the optimal sampling and DNA isolation procedures is crucial for robustness and reproducibility of the results. We p
Chao Liu3,12,13* and Mengge Wang1,3,12,14*† Abstract Background The underrepresentation of Hmong-Mien (HM) people in Asian genomic studies has hindered our comprehensive understanding of the full landscape of their evolutionary history and complex trait archit...
One of the principal advantages of the invention is the provision of simple and practical processing for recovering glycoproteins from whey resulting from the production of cheese from bovine milk. Bovine whey, typically liquid whey, is a complex mixture of protein, fat and salt components in vario...