Current protocols for inclusion body isolation from Escherichia coli usually offer between 95 to 99% of protein recovery, what in practical terms, might imply extensive bacterial cell contamination, not compatible with the use of inclusion bodies in biological interfaces. Results Using an appropriate ...
A change in the configuration of DNA occurred due to the binding of the aptamers to the cell targets, increasing the distance between the dye and the GO and subsequently recovering the fluorescence signal. As an alternative, a "turn-on" detection method using a microarray modified with rGO ...
Supplementary TableS1). The median values of the A260/A280 ratio (the measure of purity of DNA) were well within the expected range (1.8–2) and did not differ significantly between the DNA isolation kits or between the sampling kits (Fig....
Duranti (2001) An approach to the critical assess- ment of the experimental conditions in practical molecular biology: isolation of plant DNA, Biochem. Mol. Biol. Educ. 29, 21-23.Alessio Scarafoni,Marcello D.An approach to the critical assessment of the experimental conditions in practical ...
However, four of these 31 gene clusters had unknown functions, indicating that they had the potential for further exploration. Chlortetracycline, a broad-spectrum antibiotic of the tetracycline class, has inhibitory effects on both gram-positive and gram-negative bacteria and could treat livestock and...
After 12 days of incubation at 28°C, fermentation filtrates were extracted with three half-volumes of ethyl acetate (EtOAC), and the crude extracts were separated and dried using rotary evaporator at 40°C and 240 mbar.27 Phylogenetic tree full of 16S rDNA gene sequencing DNA extraction...
Isolation and separation of specific molecules is used in almost all areas of biosciences and biotechnology. Diverse procedures can be used to achieve this goal. Recently, increased attention has been paid to the development and application of magnetic s
The invention includes in vitro methods of inducing and enhancing proliferation of human marrow stromal cells for use in, for example, gene therapy and transplantation methods. The
Contamination of these ecosystems constitutes a serious environmental problem due to the high toxicity exhibited by the aromatic hydrocarbons, which belong to a class of environmentally per- sistent compounds3. Microbial degradation has been deemed as the most effective and important strat- egy for ...
2.5. DNA Extraction and Manipulation DNA from bacteriophage lysates was isolated using Phage DNA Isolation Kit (Norgen Biotek, Ontario, Canada) according to the manufacturer’s instructions. Extraction of bacterial DNA and of DNA from a smaller volume of purified and concentrated phage culture (2 ×...