Below we present explicit step by step protocols that we used in the 5-week summer class, Biology 656, at the University of South Carolina. We used these protocols to construct libraries, screen those libraries, and determine flanking sequences, in 3 birds, 3 reptiles, 2 mammals, 2 insects...
The emergence of pyrethroid resistance is a major concern for vector control strategies as it threatens the efficiency of the insecti- cide treated nets, all of which contain this insecticide class, that have proven so successful ...
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A change in the configuration of DNA occurred due to the binding of the aptamers to the cell targets, increasing the distance between the dye and the GO and subsequently recovering the fluorescence signal. As an alternative, a "turn-on" detection method using a microarray modified with rGO ...
Adipose tissue mesenchymal stem cells (ASCs) are a class of MSCs derived from the adipose-tissue stromal vascular fraction [1]. ASCs are widely used in tissue engineering and regenerative medicine. Methods of immunoaffinity-ASCs isolation are based on the binding of surface antigenic markers such...
Subsequent cycles, additionally using a solution-borne forward primer, produce duplicate copies of the double stranded DNA (dsDNA). Thus, the final PCR product is in the form of bead-immobilized dsDNA where the target-binding oligonucleotides are hybridized to their complementary strands, which are ...
With a suitable choice of gal −mutations most of the papillae arise by recombination and some of those colonies with less than normal numbers prove to be defective in some aspect of recombination or DNA repair. In addition to strains carrying mutations that can be ascribed to known loci, se...
Ding, W.Epithelial-to-mesenchymal transition of murine liver tumor cells promotes invasion.Hepatology.52, 945-953 (2010). Tags CD133+ Liver Stem CellsClonal ExpansionChronic Liver InjuryHepatocytesCholangiocytesLiver CancerHepatocellular CarcinomaStem Cell IsolationSolid OrganRare Population Of CellsPar...
Polymorphism of the MHC class I α1 domain was determined using PCR with genomic DNA from 12 fish. Sixteen variants were identified with most diversity concentrated in those amino acids that bind directly to peptides in mammalian class I molecules. Non-synonymous substitutions predominate over ...
The invention provides a method for the separation and purification of two or three cellular components selected from genomic DNA, RNA and proteins from a single biological sample.