Targeted sequencing (instead of WGS) is used when the goal of the experiment is to sequence specific genes, sets of related genes, or targeted regions of a genome. An example of targeted sequencing is screening for known cancer...
Along with this experiment, we also analyzed the distribution of the R-loop in the chloroplast genome by ssDRIP-seq30, together with the binding profile of ATH by ChIP-seq. Compared with Col-0, the strand breaks in the atrnh1c chloroplast genome were significantly increased, while the ...
The antibodies used in this experiment is LbCpf1/Cas12a Mouse mAb (Strain ND2006, 2D5-6G11, Cell Signaling Technology), MFN2 Polyclonal antibody (Proteintech, Cat #12186), HSP 60 (A57-B9, Santa Cruz Biotechnology, Cat# sc-57840) and Rabbit monoclonal anti-GAPDH antibody (14C10, Cell ...
initially interpreted as faster biological aging rates, though now thought possibly due to the impact of hormones on tissues such as the breast [182]. While cell isolation techniques may enable robust and insightful studies due to larger sample sizes, their level of resolution ...
Since the sources of RIND-EDSBs are unknown, we assumed that, besides the independent variable of each experiment, other factors that may influence RIND-EDSBs in our experiments were the same between test and control cells grown under the same condition. To analyze EDSBs present in non-...
In one experiment, they sought to disturb the gene CCR5 to make cells resistant to HIV. In the second, they created a gene mutation that can protect against blood disorders, including sickle cell disease. They observed the cells encapsulated the nanoparticles within six hours and began the gene...
However, this method was rarely able to achieve species-level identification, and application of the method to assess helminth biodiversity ultimately required worm isolation from fecal samples and amplification with class-specific primers [4]. To overcome the challenge of host DNA interference, we ...
The most commonly-used commercial DNA and RNA isolation kits are indicated in Supplementary Table 1. For example, the Qiagen's PowerSoil DNA isolation kit adapted for use with magnetic bead plates is recommended by the Earth Microbiome Project (Marotz et al., 2017). It is also considered as ...
Natural mitochondrial DNA (mtDNA) mutations enable the inference of clonal relationships among cells. mtDNA can be profiled along with measures of cell state, but has not yet been combined with the massively parallel approaches needed to tackle the compl
copies of the relevant DNA target were present in a 50 μl reaction. All readings were normalized to the no template control (NTC) at the start of the experiment. The N1 gRNA gave an unexpected result, whereby it triggered the collateral activity of AsCas12a and its variants without a ...