Rapid and practical DNA isolation from Trichomonas vaginalis and other nuclease rich protozoans - Riley, Krieger () Citation Context ...TC-3'). Trichomonad αtubulin gene was used as an internal control. The PCR was performed for 25 cycles. Genomic DNA isolation and Southern blot Genomic DNA...
a Construction and Isolation of DNA A given DNA fragment, after verification of correct cloning and confirmation of the nucleotide sequence, is purified through several steps to ensure that the DNA fragment does not contain nicks or strand breaks and is as pure as possible. For these final steps...
4.4.1 iBioChips integrate diagnostic assays and cellular engineering into miniaturized chips that achieve cutting-edge sensitivity and high-throughput. We have resolved traditional biotech challenges with innovative biochip approaches https://ibiochips.com/?gclid=Cj0KCQjwuLPnBRDjARIsACDzGL0wb6u79VHHkftod...
Environmental DNA (eDNA) refers to DNA that can be extracted from environmental samples (such as soil, water, feces, or air) without the prior isolation of any target organism. The analysis of environmental DNA has the potential of providing high-throughput information on taxa and functional gene...
Controlling the false discovery rate: a practical and powerful approach to multiple testing. J. R. Statist. Soc. B 57, 289–300 (1995). Google Scholar Cibulskis, K. et al. Sensitive detection of somatic point mutations in impure and heterogeneous cancer samples. Nat. Biotechnol. 31, 213...
The study of global biodiversity will greatly benefit from access to comprehensive DNA barcode libraries at continental scale, but such datasets are still very rare. Here, we assemble the first high-resolution reference library for European butterflies t
Isolation DNA from induced culture followed by electroporation into E. coli was performed by using the alkaline lysis method, which would denature chromosome DNA. Normally, 50 ~ 100 transformants would appear on ampicillin plus apramycin LB plate for every single electroporation. Twenty colonies ...
Genetic engineering of crop plants has been successful in transferring traits into elite lines beyond what can be achieved with breeding techniques. Introduction of transgenes originating from other species has conferred resistance to biotic and abiotic
These studies are not supported by phage isolation or electron microscopical evidence. Most environmental phages have been isolated after enrichment and are therefore not representative of the environment. Electron microscopical studies of nonbiased, uncultured phage populations are much rarer and, generally...
The DNA is then hybridized to a whole-genome array to identify its genomic location (FIG. 3). Sequence- specific isolation of insertion sites has been successfully demonstrated using oligonucleotide capture probes75, or by annealing linkers to fragmented DNA and using PCR81 by adapting a ...