4.4.1 iBioChips integrate diagnostic assays and cellular engineering into miniaturized chips that achieve cutting-edge sensitivity and high-throughput. We have resolved traditional biotech challenges with innovative biochip approaches https://ibiochips.com/?gclid=Cj0KCQjwuLPnBRDjARIsACDzGL0wb6u79VHHkftod...
Controlling the false discovery rate: a practical and powerful approach to multiple testing. J. R. Statist. Soc. B 57, 289–300 (1995). Google Scholar Cibulskis, K. et al. Sensitive detection of somatic point mutations in impure and heterogeneous cancer samples. Nat. Biotechnol. 31, 213...
This file describes the detailed protocols of tissue fixation, sonication and DNA extraction, including several steps: pulverization of tissue, cross-linking of tissues, sonication of tissues and DNA isolation (precipitation and chromatin fragmentation check). Supplementary Data This is a compressed file...
(2) Preparation of nuclei suspensions: freshly extracted liver biopsies were cut into small pieces and transferred into a 1.5–2 ml tube containing nucleus isolation buffer (NaCl 146 mM, Tris-HCl 10 mM, CaCl2 1 mM, MgCl2 21 mM, bovine serum albumin 0.05%, Nonidet P-40 0.2% ...
Burnham KP, Anderson DR: Model selection and multi-model inference: A practical information-theoretic approach. 2002, New York, NY: Springer-Verlag, 488-2 Google Scholar Paetkau D: An empirical exploration of data quality in DNA-based population inventories. Molecular Ecology. 2003, 12 (6):...
immunoblot (WB) or immunofluorescence assay (IFA) or one of several virological (i.e. non-antibody) tests: either HIV nucleic acid, HIV p24 antigen with neutralization, or HIV-1 isolation by viral culture.35 Although the EI assays are highly sensitive and specific, the positive predictive ...
Total RNA was extracted using a mirVana RNA isolation labelling kit (Ambion). We used 500 ng of total RNA for labelling and hybridization based on the manufacturer's procedures (Illumina). Sentrix Human6 v2 Expression Bead Chip and HumanHT-12 v4 Expression Beadchip were used. The bead chips ...
Alessio Scarafoni,Marcello D.An approach to the critical assessment of the experimental conditions in practical molecular biology:isolation of plant DNA.Biochemistry and Molecular Biology Education. 2001An approach to the critical assessment of the experimental conditions in practical molecular biology: ...
4. AN ARCHITECTURE FOR SPATIALLY ISOLATED DNA GATES We propose a practical approach to DNA-based computation that takes advantage of spatial isolation and organization. In our ap- proach, DNA structures that implement computational elements, such as logic AND and OR gates, are attached to a "...
RNA IsolationOocyte Injections: Injection of mRNA or cRNA into Xenopus oocytes was done by a modification of established protocols (A. Coleman, Transcription and Translation: A Practical Approach. B. D. Hanes, S. J. Higgins, Eds., 1984); Y. Masu, et al., Nature 329, 836-838 (1987)...