Isolation and culture of primary hepatocytes from resected human liver tissue. Methods Mol. Biol. Clifton N. J. 640, 57e82.Lecluyse EL, Alexandre E (2010) Isolation and culture of primary hepatocytes from resected human liver tissue. Methods Mol Biol 640: 57-82....
Primary hepatocyte culture is a valuable tool that has been extensively used in basic research of liver function, disease, pathophysiology, pharmacology and other related subjects. The method based on two-step collagenase perfusion for isolation of intact hepatocytes was first introduced by Berry and ...
Hepatocytes comprised 93.4% of the total cell isolate. Survival of hepatocytes in suspension culture was dependent on fetal bovine serum concentration and temperature of incubation. Serum concentrations of 5, 10, and 20% produced the highest survival during primary culture. Hepatocyte survival was in ...
Isolation and Culture of Primary Hepatocytes from Resected Human Liver Tissue As our knowledge of the species differences in drug metabolism and drug-induced hepatotoxicity has expanded significantly, the need for human-relevant in v... Edward L Lecluyse,E Alexandre - 《Methods in Molecular Biology...
None of the cell lines grew in semi-solid agar, nor did they form tumours in nude mice, suggesting they are non-tumourigenic. These novel murine oval cell lines, together with a reliable method for isolation and culture of primary oval cells, will provide a useful tool for investigating ...
Objectives To investigate the metabolism of Ost in rat primary hepatocytes incubation system, analysis the metabolic mechanism and character of Ost and the drug interactions. 目的 采用大鼠原代肝细胞体外温孵液对蛇床子素(Ost)在肝细胞中代谢转化过程进行研究。 更多例句>> 4) Primary culture of murine ...
Primary hepatocyte cultures better reflect the properties of the liver in vivo than do cell lines derived from the liver. Here we describe a method for the isolation and culture of mouse primary hepatocytes. The cells are viable, can be transfected by DNA, and retain key properties of liver ...
兔骨髓间充质干细胞的分离培养及肝内示踪 张芙蓉屠慧渭钱波孙何金立方 绍兴文理学院生命科学学院,浙江绍兴312000 摘要:通过分离培养兔骨髓来源的间充质干细胞(MSCs),示踪其肝内移植命运,为MSCs的细胞治 疗肝脏疾病提供理论依据和试验支持。采取全骨髓贴壁培养法分离培养兔骨髓MSCs,利用携带增强型绿 色荧光蛋白(EGFP)...
The major limitation of using primary hepatocytes is the rapid dedifferentiation of hepatocytes during isolation and culture, whereby most of the liver-specific functions are lost. Despite the many efforts that have been made to optimize the culture condition, current primary cultured hepatocyte preparati...
Hepatic progenitor cells Hepatic differentiation Hepatoblasts Hepatocytes Human pluripotent stem cells Serum-free culture 1. Introduction Primary hepatocytes are used as a tool for predicting drug-induced hepatotoxicity during drug development. However, their limited growth potential, narrow ranges of sources...