Here we describe a method for the isolation and culture of mouse primary hepatocytes. The cells are viable, can be transfected by DNA, and retain key properties of liver cells such as the induction of cytochrome P450 gene expression by drugs such as phenobarbital....
Primary hepatocyte cultures better reflect the properties of the liver in vivo than do cell lines derived from the liver. Here we describe a method for the isolation and culture of mouse primary hepatocytes. The cells are viable, can be transfected by DNA, and retain key properties of liver ...
3) rat primary hepatocyte 大鼠原代肝细胞 1. Objectives To investigate the metabolism of Ost in rat primary hepatocytes incubation system, analysis the metabolic mechanism and character of Ost and the drug interactions. 目的 采用大鼠原代肝细胞体外温孵液对蛇床子素(Ost)在肝细胞中代谢转化过程进行...
Aim : Embryonic stem (ES) cells are being widely investigated as a promising source of hepatocytes with their proliferative, renewable, and pluripotent cap... S Fang,YD Qiu,L Mao,... - 《中国药理学报》 被引量: 51发表: 2010年 Hepatoblast-like cells enriched from mouse embryonic stem cells...
Hepatocytes comprise the majority of liver mass and cell number. However, in order to understand liver biology, the non-parenchymal cells (NPCs) must be considered. Herein, a relatively rapid and efficient method for isolating liver NPCs from a mouse is described. Using this method, liver sinuso...
Isolation and Culture of Adult Mouse Hepatocytes The liver performs a multitude of functions including the regulation of carbohydrate, fat, and protein metabolism, the detoxification of endo- and xenobiot... WC Li,KL Ralphs,D Tosh - Humana Press 被引量: 224发表: 2010年 Primary cultures and th...
A correction was made to: Isolation of Human Hepatocytes by a Two-step Collagenase Perfusion Procedure The changes listed below have been made to Table 1. 1). In the recipe for Solution 2, the Final Concentration of EGTA has been changed from: EGTA 0.1mM to: EGTA 1mM 2). In the reci...
The liver performs a multitude of functions including the regulation of carbohydrate, fat, and protein metabolism, the detoxification of endo- and xenobiotics, and the synthesis and secretion of plasma proteins and bile. Isolated hepatocytes constitute a useful technique for studying liver function in ...
Primary cells that do not proliferatein vitro,such as hepatocytes, should be cryopreserved in aliquots immediately after recovery, leaving aside only what will be immediately used. Extra caution needs to be taken when freezing down primary cells, and especially non-proliferating cells ...
Hepatic differentiation of BMOL-TAT cells could be traced by measuring the expression of their lacZ transgene, which is driven by a promoter element from tyrosine aminotransferase (TAT), a marker of adult hepatocytes. Interestingly, haematopoietic markers were upregulated in superconfluent cultures, ...