Because an HBV-permissible mouse model would be helpful for the study of HBV pathogenesis, it is necessary to investigate whether human NTCP supports the susceptibility of mouse hepatocyte cell lines to HBV. The results show that exogenous human NTCP expression can render non-susceptible HepG2 (...
DescriptionThe Immortalized Mouse Hepatocyte SIMHCLOCKCells contain a bioluminescent marker gene that reports on the circadian phase of the cells. The presence of the Per2:Luc reporter gene allows real time monitoring of clock phase. The major characteristics of this cell line include its high metabo...
The aim of this study was to determine the role of the antioxidant glutathione in this process.A rat hepatocyte cell line and primary hepatocytes sensitized to TNF-alpha toxicity by the addition of actinomycin D were examined for changes in glutathione levels and for the effects of glutathione ...
Risal P,Cho BH,Sylvester KG,et al.The establishment and charac-terization of immortal hepatocyte cell lines from a mouse liver injurymodel[J].In Vitro Cell Dev Biol Anim,2011,47(8):526-534.The establishment and characterization of immortal hepatocyte cell lines from a mouse liver injury ...
In conclusion, our in vitro and in vivo experiments revealed that cultured EBs contain functional hepatocytes or hepatocyte-like cells. (HEPATOLOGY 2002;36:22-29.)Access through your organization Check access to the full text by signing in through your organization. Access through your organization...
2.2. Cell culture AML-12, a non-transformed mouse hepatocyte cell line was obtained from American Type Culture Collection (ATCC, Manassas, VA). AML-12 cells were cultured in Dulbecco’s Modified Eagle Medium/Ham’s Nutrient Mixture F-12, 1:1 (DMEM/F-12, Hyclone) containing 10% (v/v)...
Methods: After hepatocyte growth factor (HGF), p-nerve growth factor (NGF) and retinoic acid (RA) were added into cell culture respectively, and co-culture with mouse embryonic hepatocytes, the morphological changes of the mouse ES cells were observed; albumin and transthyretin mRNA were examined...
The promotion of hepatic maturation of human pluripotent stem cells in 3D co-culture using type I collagen and Swiss 3T3 cell sheets Hepatocyte-like cells differentiated from human embryonic stem cells (hESCs) or human induced pluripotent stem cells (hiPSCs) are known to be a useful cell... ...
Cell lines and culturing The mouse liver hepatocyte cell line AML-12 [established from hepatocytes from a male mouse (CD1 strain, line MT42) non-tumorigenic], preserved by our laboratory, was cultured according to the ATCC guideline, with 10% fetal bovine serum (Gibco, United States) added ...
protein ofMHV-A59 may not be cleaved by these cell types. Indeed, the MHV-A59 S pro- tein was not cleaved when the virus replicated in primary hepatocyte or glial cell cultures, although it is cleaved by L2 cells. Western blot analysis, therefore, is consistent with the observation that...