Isolation of primary human hepatocytes from normal and diseased liver tissue: A one hundred liver experience . PLoS ONE 6 , e18222Bhogal RH, Hodson J, Bartlett DC, Weston CJ, Curbishley SM, et al. (2011) Isolation of primary human hepatocytes from normal and diseased liver tissue: a one ...
Research and therapeutic applications create a high demand for primary human hepatocytes. The limiting factor for their utilization is the availability of metabolically active hepatocytes in large quantities. Centrifugation through Percoll, which is commonly performed during hepatocyte isolation, has so far ...
Xenobiotics are primarily metabolized by hepatocytes in the liver, and primary human hepatocytes are the gold standard model for the assessment of drug efficacy, safety, and toxicity in the early phases of drug development. Recent advances in single-cell genomics demonstrate liver zonation and ploidy ...
Primary human hepatocytes (PHHs) are considered the gold standard for thein vitrostudy ofHBVreplication as they directly reflect the metabolism and functionality of the human liver. However, several limitations of this system include PHH donor-to-donor variability, limited life span and low permissiv...
Xenobiotics are primarily metabolized by hepatocytes in the liver, and primary human hepatocytes are the gold standard model for the assessment of drug efficacy, safety, and toxicity in the early phases of drug development. Recent advances in single-cell
Isolation and Culture of Primary Human Hepatocytes As our knowledge of the species differences in and drug-induced has expanded significantly, the need for -relevant in vitro hepatic model systems has becom... EL Lecluyse,E Alexandre,GA Hamilton,... - 《Methods in Molecular Biology》 被引量...
(85.12±2.87)%,and(91.84±30.3)%;the consumed amount of collagenase was respectively(17.38±2.13),(78.75±20.83) and(31.2±12.46)mg.Conclusion The improved collagenase perfusion isolation method is a economical one with high efficiency for rat hepatocytes compared to tissue and collagenase perfusion ...
BMC Endocrine Disorders (2024) 24:135 https://doi.org/10.1186/s12902-024-01663-9 BMC Endocrine Disorders RESEARCH Sex hormones differently regulate lipid metabolism genes in primary human hepatocytes Open Access Lena Seidemann1,2, Clara Paula Lippold1,2, Carolin Marie Rohm1,2, Julian ...
Isolation and primary culture of adult human hepatocytes Biopsy tissue of adult human liver was gently dissociated with collagenase followed by Dispase. By repeated low g centrifugation, a large number of almost pure, viable hepatocytes was obtained. This is the first report of a successful pr......
Enrichment of hepatic progenitor cells from adult mouse liver Hepatic progenitor cells (HPCs) have been characterized in several drug-treated rodent models and in the fetal liver; however, their properties have not be... - 《Hepatology》 被引量: 217发表: 2003年...