L. WOLIN, 1998 A role for the yeast La protein in U6 snRNP assembly: evidence that the La protein is a molecular chaperone for RNA polymerase III transcripts. Embo J 17: 7442-7453.Pannone, B. K., D. Xue and S. L. Wolin, 1998 A role for the yeast La protein in U6 snRNP ...
LC3/ATG8 has long been appreciated to play a central role in autophagy, by which a variety of cytoplasmic materials are delivered to lysosomes and eventually degraded. However, information on the molecular functions of LC3 in RNA biology is very limited.
mRNA biogenesis in the eukaryotic nucleus is a highly complex process. The numerous RNA processing steps are tightly coordinated to ensure that only fully processed transcripts are released from chromatin for export from the nucleus. Here, we present the
Rna CambridgeAnthony, J.G., Weidenhammer, E.M. and Woolford, J.L., Jr. (1997) The yeast Prp3 protein is a U4/U6 snRNP protein necessary for integrity of the U4/U6 snRNP and the U4/U6.U5 tri-snRNP. RNA, 3, 1143-1152.
RNA binding proteins (e.g. FUS, ADAR, Quaking, MBL, etc.), known to impact splicing regulation or RNA editing, influence circRNAs production [8–11], some of which may act analogously as flanking complementary sequences. For example, the RNA binding protein Quaking (QKI) promotes RNA ...
Here, we identified SF3b1 as a Tat-SF1–interacting subunit of the U2 snRNP. Our 1.1 Å resolution crystal structure revealed that Tat-SF1 contains a U2AF homology motif (UHM) protein–protein interaction module. We demonstrated that Tat-SF1 preferentially and directly binds the SF3b1 ...
Gene expression includes the synthesis of RNA from DNA by RNA polymerase and synthesis of protein from RNA by the translation process. These two processes are under strict regulation of multiple factors regulated by various processes that dictate the syn...
These results suggest that human SRP 7SL RNA adenylating enzyme lacks substrate specificity, at least on naked RNAs, and can function as a processive poly(A) polymerase; it is thus unlikely to play any role in U2 snRNA metabolism. CCA Addition to U2 snRNPs Mature U2 snRNP consists of a ...
A universal characteristic of eukaryotic transcription is that the promoter recruits RNA polymerase II (RNAPII) to produce both precursor mRNAs (pre-mRNAs) and short unstable promoter upstream transcripts (PROMPTs) toward the opposite direction. However, how the transcription machinery selects the correct...
The biogenesis of spliceosomal small nuclear RNAs (snRNAs) involves organized translocations between the cytoplasm and certain nuclear domains, such as Cajal bodies and nucleoli. Here we identify human RBM28 protein as a novel snRNP component, based on a