Gene expression was analysed by qRT-PCR and retinal ultrastructure examined by transmission electron microscopy. Single cell sorting of control and patient iPSCs and ROs was performed to capture individual cells which were then subjected to RNA-Seq and bioinformatic analysis. Results : A patient-...
there has been limited success in targeting surface antigens in AML, in part due to shared expression across malignant and normal cells. Here, high-density immunophenotyping of AML coupled with proteogenomics identified unique expression of a variety of antigens, including the RNA helicase U5 snRN...
Mechanistically, YF1 increased the mRNA expression ratio of IL-10/pre IL-10 by binding to SNRNP200, a spliceosome-specific protein for pre-mRNA splicing, with reduced SNRNP200 ubiquitination. And it was reversed by Brr2-IN-3, a specific SNRNP200 inhibitor. Collectively, we hold that YF1 ...
DNA screening was carried out by exon-PCRs and Sanger sequencing. For 4 patients, we obtained lymphoblastoid cell lines following immortalization of circulating lymphocytes with the Epstein-Barr virus. Gene expression was tested by relative and allele-specific qPCR, at the steady state and after ...
Knocking down Snrnp200 in zebrafish triggered aberrant splicing of the cbln1 gene, upregulation of other U4/U6-U5 tri-snRNP components, and downregulation of a panel of retina-specific transcripts. Systemic defects were found correlated with knockdown of Snrnp200 in zebrafish. Only demorphogenesis ...
Prognostic Significance of Elevated UCHL1, SNRNP200, and PAK4 Expression in High-Grade Clear Cell Renal Cell Carcinoma: Insights from LC-MS/MS Analysis and Immunohistochemical ValidationRETINITIS pigmentosaCELL proliferationMANN Whitney U TestDESCRIPTIVE statisticsGENE expression...