U1 A protein can also bind the loop sequence when it is part of an artificial RNA which cannot form a stable hairpin structure. The region of the protein required to bind to U1 snRNA consists of a conserved 80 amino acid motif, previously identified in many ribonucleoprotein (RNP) proteins,...
The release of RNAP II is mediated by phosphorylation through P-TEFb that in turn is under control by the inhibitory 7SK small nuclear ribonucleoprotein (snRNP) complex. The 7SK snRNP consists of the 7SK non-coding RNA and the proteins MEPCE, LARP7, and HEXIM1/2. Biallelicloss-of-...
The cis elements comprise splicing enhancers and silencers that can be located in either the exons or the introns and that bind activator and repressor proteins. Sometimes, the presence or absence of a single regulator is sufficient to determine alternative splicing pathways. More commonly, ...
The spliceosome consists of small nuclear RNA proteins (snRNPs) U1, U2, U4, U5 and U6, together with approximately 80 conserved proteins. U5 snRNP contains nine specific proteins. This gene encodes one of the U5 snRNP-specific proteins. This protein belongs to the DEXH-box family of putative...
RNA-binding proteins Ribosome tRNAs Introduction The superphylum Alveolata diverged approximately 850 million years ago into two main branches: ciliates and myzozoans. The Myzozoa, which includes Apicomplexa, Chromerida, Perkinsozoa, and Dinoflagellates, consists of obligate intracellular parasites and...
This complex was isolated by immunoaffinity against SKIP, a protein that joins the spliceosome concomitant with the U4/U6-U5 triple-snRNP. Interestingly, anti-SKIP antibodies also pull down a 35S complex containing U5 snRNA and a distinct set of proteins that includes splicing factors associated ...
hundreds of interacting proteins and small nuclear RNAs (snRNAs) and a number of small nuclear ribonucleoproteins (snRNPs). In the absence of mutations, the process of splicing is highly ordered and precise, involving several multi-component splicing factors with the addition of the above mentioned ...
In mammals, the circadian clock consists of a cell-autonomous transcription-translation feedback loop that temporally regulates the expression of a large number of genes. Heterodimers of the CLOCK (or NPAS2) and BMAL1 proteins drive the expression of their own repressors, CRY (CRY1 and CRY2)...
consisted of several ChIP-seq datasets, and provided evidence that KAP1, also known as TRIM28, acts as a scaffold to recruit the 7SK snRNP complex to gene promoters to facilitate productive transcription elongation in response to stimulation. Their bioinformatics analysis showed that 70% of all ...
These domains have been shown, by truncation and domain deletion in the closely related protein RBM5, to be important for spliceosome assem- bly and recruitment to pre-mRNA [24] In particular, it has been shown that the OCRE domain binds to the C- terminal tail of snRNP core proteins [...