In the absence of their acid/base residue, thioglycoligases require both an activated glycosyl donor to form the glycosyl-enzyme intermediate and an acceptor that is a strong nucleophile, capable of reacting without basic catalytic assistance (Fig. 1a); consequently, nucleophiles successfully assayed ...
An SN2 reaction in organic chemistry is a type of substitution reaction that occurs in a single step with the use of a strong nucleophile. During an...Become a member and unlock all Study Answers Start today. Try it now Create an account Ask a question Our experts can answer your toug...
What is the purpose of adding NaOH solution to the initial organic phase? Explain why KNO_3 will not dissolve in hexane (C_6H_{14}) ? In gas chromatography (G.C.), what is the purpose of injecting the sample onto a heated block? What is the purpose of adding concentrated HCl to th...
<p>To understand why fluorine is the most reactive halogen, we can break down the explanation into several key points:</p><p>1. <strong>Identify the Halogens</strong>: - The halogens include fluorine (F), chlorine (Cl), bromine (Br), iodine (I), and a
In CGI-58, Asn155 replaces the putative active site nucleophile. A large, primarily helical lid region (Pro180–Leu280) is inserted after strand β6 and covers the potential “active site.” An additional feature of the model is a long partly helical, partly unstructured region ranging from...
Because everynucleophileis potentially abase, and vice versa. If you have a reaction where it looks like you might get SN2 or E2, look closely first – is there any chance of a simple acid-base reaction? For instance, take NaOH plus an alkyl thiol, R–SH. Is it an SN2? Or possibly...
optionally in the presence of a suitable base (e.g. NaOH, triethylamine, pyridine or potassium carbonate) and an appropriate solvent (e.g. ether, water, dichloromethane, THF, tert-butyl methyl ether, or mixtures thereof). Compounds of formulae IV, VI, VIII, IX, X, M, XIII and XIV ...
Identify the stronger nucleophile in the following pair. A. CH_3OH B. CH_3NH_2Which of the following is the strongest acid? a) H_3PO_4 b) NH_4^+ c) NaOH d) H_2CO_3 e) HCl1. Which of the following is the strongest acid? a) CH4 b) NH3 c) H2O d) ...
Cysteine functions as a catalytic nucleophile, while glutamate activates a water molecule in the deacylation step [15]. The motif of the A. geothermalis ALDH is Gly-X-Gly-X-X-Gly (GXGXXG). For this A. geothermalis ALDH, the linear sequence closely resembles the motif, Pro-Leu-Gly-Val...