Nu: nucleophile. b Overlap of five solved structures from the GH3 family and modeled rBxTW1 showing the three conserved acid residues of the active site and a β-d-glucose ligand. E: Catalytic acid/base (Glu); D: Catalytic nucleophile (Asp); E/D: Conserved acid residue (Glu for β-...
NaOH solution hydrolyzes tert-butyl bromide, an example of an SN1 reaction. The pace of the reaction relies on the concentration of tert-butyl bromide, but the concentration of NaOH does not affect it. As a result, just tert-butyl bromide is required to determine the rate. It is possible ...
The laboratory synthesis of isopentyl diphosphate, the building block of the molecule used for constructing isoprenoid molecules such as cholesterol, etc was accomplished by conversion of alcohol to organic tosylate, and then displacing the tosylate group with inorganic pyrophosphate nucleophile....
Why is KI a better nucleophile than KCl? Why is the carbonate solution boiled before titrating? Why is bromothymol blue a good indicator with which to test all solutions first? Why there no color change in Glycine when mixing with HNO_3 and NaOH? Why is ClO- named hypochlorite? Why do ...
specifically, 50 mM sodium phosphate (Na2HPO4and NaH2PO4), 150 mM NaCl. The pH was adjusted at pH=7.4 and pH=12.8. The final pH value in each solution was adjusted by adding the required amounts of NaOH solutions. Measuring solutions were filtered through a 0.2-μm membrane before eac...
Here we bring extensive bioinformatics, in vivo and in vitro experimentation to compose a unified view of the biosynthesis of these widely distributed non-canonical amino acids that both derive by pyridoxal-mediated β-elimination of the activated O-phosphorylated substrates followed by β-addition of...
Because every nucleophile is potentially a base, and vice versa. If you have a reaction where it looks like you might get SN2 or E2, look closely first – is there any chance of a simple acid-base reaction? For instance, take NaOH plus an alkyl thiol, R–SH. Is it an SN2? Or ...