What is the purpose of adding 40% NaOH solution to the tin reduction mixture ? What is the purpose of hydrogen peroxide in the cobalt complex lab ? What would be the benefit of having a limiting reagent when performing a lab experiment? Why not simply make both reactants go to completion...
Nu: nucleophile. b Overlap of five solved structures from the GH3 family and modeled rBxTW1 showing the three conserved acid residues of the active site and a β-d-glucose ligand. E: Catalytic acid/base (Glu); D: Catalytic nucleophile (Asp); E/D: Conserved acid residue (Glu for β-...
NaOH solution hydrolyzes tert-butyl bromide, an example of an SN1 reaction. The pace of the reaction relies on the concentration of tert-butyl bromide, but the concentration of NaOH does not affect it. As a result, just tert-butyl bromide is required to determine the rate. It is possible ...
The EcBcsGΔN activity assay was performed in 96-well microtiter plates by mixing purified EcBcsGΔN (50 μm) with p-NPPE (7 mm) in assay buffer (50 mm HEPES:NaOH (pH 7.5) and 50 mm NaCl) with addition of cello-oligosaccharides (3–5 mm), as specified, in final volumes of 100...
specifically, 50 mM sodium phosphate (Na2HPO4and NaH2PO4), 150 mM NaCl. The pH was adjusted at pH=7.4 and pH=12.8. The final pH value in each solution was adjusted by adding the required amounts of NaOH solutions. Measuring solutions were filtered through a 0.2-μm membrane before eac...
Because everynucleophileis potentially abase, and vice versa. If you have a reaction where it looks like you might get SN2 or E2, look closely first – is there any chance of a simple acid-base reaction? For instance, take NaOH plus an alkyl thiol, R–SH. Is it an SN2? Or possibly...