a) NaCl and NaNO_3 b) HCl and NaOH c) H_2CO_3 and KHCO_3 d) NaCl and NaOH e) H_2O and HCl Which of the following represent a buffer system? check all that apply A. NaOH and NaCl B. H2CO3 and NaHCO3 C. HF and KF ...
Fixed embryos were homogenized by douncing in an ice cold A1 buffer (15 mM HEPES (pH 7.5), 15 mM NaCl, 60 mM KCl, 4 mM MgCl, 0.5% Triton X-100, 0.5 mM DTT, protease inhibitors) and A2 buffer (15 mM HEPES (pH 7.5), 140 mM NaCl, 1 mM EDTA, 0.5 mM...
Recombinant active ERK1 was incubated in a kinase reaction buffer with bacterially-purified GST-tagged CDK12 fragments (F1, F2, F3, and F4) (Fig. 2d). We found that activated ERK1 robustly increased [32P] label incorporation into purified GST-CDK12 F2, but not into F1, F3, and F4 (...
At time of assay, cells were lysed in the appropriate volume of lysis buffer (10 mM HEPES, 1 mM EDTA, 100 mM NaCl, 5 mM MgCl, 142.5 mM KCl, and 1 mM DTT) so that equal cell number/unit volume was obtained for each sample. Samples were incubated on ice for 45 minutes followed ...
Cells were lysed with buffer A (10 mM HEPES [pH 7.9], 10 mM KCL, 0.1 mM EDTA, 0.1 mM EGTA, 1 mM DTT, and 1% Nonidet P40) followed by centrifugation. The pellet was treated with buffer B (20 mM HEPES [pH 7.9], 0.4 M NaCl, 1 mM EDTA, 1 mM EGTA, and 0.1 mM PMSF) to ...
A 10% homogenate from rat hearts or P19 cells was prepared in buffer (0.25 M sucrose, 0.145 M NaCl, 10 mM Tris-HCl, pH 7.4) and centrifuged for 10 min at 600 × g (Beckman J2-H with JA-20 rotor). The resulting pellet was washed once, resuspended in 5 ml buffer by 15 strokes...
The cells were rinsed in cold PBS, scraped in 1 ml lysis buffer (50 mM Tris-HCl [pH 7.6], 150 mM NaCl, 10% glycerol, 1 mM EDTA, 5 mM β-mercaptoethanol, 1 × complete protease inhibitor mix, and 1 × PhosSTOP phosphatase inhibitor mix [Roche]), and then disrupted by sonication....
Water is not only a powerful drink but also a universal solvent. As such, it can dissolve and absorb molecules from several substances. These dissolved particles in a volume of water are the total dissolved solids(TDS)level. TDS has two forms: organic and inorganic. Understanding ...
Animals were cultured in plastic containers or petri dishes for experiments, in 1x Montjuic water (1.6 mmol/l NaCl, 1.0 mmol/l CaCl2, 1.0 mmol/l MgSO4, 0.1 mmol/l MgCl2, 0.1 mmol/l KCl and 1.2 mmol/l NaHCO3 prepared in Milli-Q water) at 20 °C in the ...
Static insulin secretion assays on isolated islets were performed following 1 h incubations with 2 mM and 16.7 mM glucose in HEPES-supplemented KRB buffer containing 135 mM NaCl, 4.7 mM KCl, 1.2 mM KH2PO4, 5 mM NaHCO3, 1.2 mM MgSO4.7H2O, 1 mM CaCl2, 10 mM...