Tape (Fermoflex and Elastoplast, Instruvet) was used to cover and secure the catheter. From d 11 at 1500 onwards, a solution of [13C]urea (99.0 atom%; Cambridge Isotope Laboratories; 1 mg/g dissolved in sterile 0.15 mol/L NaCl) was continuously infused at 0.11 mmol/h into the right ...
In primary antibody solutions, the following antibodies were diluted to 1:100 with a blocking solution [100 mM Tris-HCl, 150 mM NaCl and 0.5% (w/v) blocking reagent (Sigma-Aldrich, St. Louis, MO, USA)]: anti-α-tubulin mouse antibody (Sigma-Aldrich: T6199), anti-H3K9me2 mouse...
Write the Henderson-Hasselbalch equation for a propanoic acid solution (CH_3CH_2CO_2H, pK_{a} = 4.874) using the symbols HA and A^-, and the given pK_{a} value for propanoic acid in the expression. What is a buffer? Which of the following mixtures could work as a...
Why is the product of saponification a salt? Why are chemical reactions useful? Why is the bicarbonate in blood an effective buffer when its pKa is 6.1, while the pH of the blood is 7.4? Why do redox reactions occur? Why does NaCl dissolve in water?
Primary and secondary antibodies were diluted in TBS-T (15.23 mM Tris-HCl, 4.62 mM Tris-Base, 150 mM NaCl, 0.1% Tween-20 adjusted to pH 7.6) with 3% nonfat milk (Table 2). Proteins were visualized with Clarity Western ECL Substrate (Cat# 1705061, BioRad) and imaged on a GE ...
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Figure 3. Calibration curve of pH nanosensor and pH measurements in cells. (a) In vitro calibration of triple-labeled pH nanosensor performed in buffer. Ratiometric measurements of the nanosensor are related to pH of the buffer and fitted to Equation (1). Mean ± SD are presented. (b) Nano...
Protein isolation and Western blotting. Protein extracts from embryonic and adult hearts were obtained by mechanical disruption of the tissue using the MagNA Lyser and MagNA Lyser beads (Roche Diagnostics) in RIPA buffer (50 mM Tris-HCl, 150 mM NaCl, 1% NP-40, 0.2% sodium deoxycholate...
For protein isolation, 30–50 mg of the tissue samples were mixed with 800 µL radioimmunoprecipitation assay (RIPA) complete buffer—containing 150 mM NaCl, 1% NP-40, 1% Natrium-Deoxycholate, 25 mM TrisHCL (pH 7.6), a phosphatase inhibitor cocktail (PhosSTOP; Roche, Mannheim, Germany), ...
The following three isoforms, containing inhibitor of trypsin - like were eluted by stepwise gradient of NaCl with different molarity: the 3rd isoform - 0.1 M NaCl, the 4th - 0.2 M NaCl, the 5th - 0.5 M NaCl. DEVELOPMENT OF A NEW ANTIVIRAL MEDICATION FOR THE GRIPPE The topics of molarity...