In primary antibody solutions, the following antibodies were diluted to 1:100 with a blocking solution [100 mM Tris-HCl, 150 mM NaCl and 0.5% (w/v) blocking reagent (Sigma-Aldrich, St. Louis, MO, USA)]: anti-α-tubulin mouse antibody (Sigma-Aldrich: T6199), anti-H3K9me2 mouse...
Write the Henderson-Hasselbalch equation for a propanoic acid solution (CH_3CH_2CO_2H, pK_{a} = 4.874) using the symbols HA and A^-, and the given pK_{a} value for propanoic acid in the expression. What is a buffer? Which of the following mixtures could work as a...
Why is the product of saponification a salt? Why are chemical reactions useful? Why is the bicarbonate in blood an effective buffer when its pKa is 6.1, while the pH of the blood is 7.4? Why do redox reactions occur? Why does NaCl dissolve in water?
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Primary and secondary antibodies were diluted in TBS-T (15.23 mM Tris-HCl, 4.62 mM Tris-Base, 150 mM NaCl, 0.1% Tween-20 adjusted to pH 7.6) with 3% nonfat milk (Table 2). Proteins were visualized with Clarity Western ECL Substrate (Cat# 1705061, BioRad) and imaged on a GE ...
The following three isoforms, containing inhibitor of trypsin - like were eluted by stepwise gradient of NaCl with different molarity: the 3rd isoform - 0.1 M NaCl, the 4th - 0.2 M NaCl, the 5th - 0.5 M NaCl. DEVELOPMENT OF A NEW ANTIVIRAL MEDICATION FOR THE GRIPPE The topics of molarity...
The cells were collected and lysed by a freeze/thaw cycle and sonication. The lysate was passed through a nickel-Sepharose column (GE Healthcare); the protein not bound to nickel-Sepharose was washed with washing buffer (0.1 m Tris-HCl, pH 7.8, containing 0.5 m NaCl, and 40 mm imidazole...
Protein isolation and Western blotting. Protein extracts from embryonic and adult hearts were obtained by mechanical disruption of the tissue using the MagNA Lyser and MagNA Lyser beads (Roche Diagnostics) in RIPA buffer (50 mM Tris-HCl, 150 mM NaCl, 1% NP-40, 0.2% sodium deoxycholate...
To quantify apoptosis, cells were spun, washed in PBS, and incubated with 3 μg/ml His-GFP-annexin-V or His-CHERRY-annexin-V and 5 μg/ml propidium iodide in annexin V binding buffer (10 mm Hepes/NaOH, pH 7.4, 140 mm NaCl, 2.5 mm CaCl2) at room temperature for 15 min. 2 × ...
In making a specific galvanic cell, explain how one determines which electrodes and solutions to use in the cell. Why do we test the metals in water when comparing ions? Why is the hydrated hydrogen ion important? Explain the differences in conductivity of NaCl, HCl, and KOH...