How much sodium bicarbonate is needed to neutralize HCl? How do you make a carbonate-bicarbonate buffer? Is ammonium bicarbonate safe? Does carbonated water add CO2 to the bloodstream? How much does a cup of sodium bicarbonate weigh? How many grams of sodium bicarbonate in a teaspoon?
Given the balanced equation NaHCO_3 + HCl \to NaCl + CO_2 + H_2O, what is the expected mole ratio of NaHCO_3 reacted to NaCl produced? What is the pH of a solution that contains 1m acetic acid and 1m sodium acetate? What is the product of the following reaction? A, B,...
Biotin Assay using Eu-SA and time-resolved fluorometry For the SABA, instead of the HEPES buffer described previously for the ABA (2), the assay buffer was 50 mmol/L Tris-HCl, pH 7.8, 154 mmol/L NaCl, 7.7 mmol/L NaN3, 0.02 mmol/L diethylene-triamine pentaacetic acid, 0.5g/L BIgG ...
Figure 3. Calibration curve of pH nanosensor and pH measurements in cells. (a) In vitro calibration of triple-labeled pH nanosensor performed in buffer. Ratiometric measurements of the nanosensor are related to pH of the buffer and fitted to Equation (1). Mean ± SD are presented. (b) Nano...
The GlyT1i (SSR504734), D-serine, and rolipram were purchased from Sigma Aldrich. SSR504734 (2.5, 10 and 40 mg/kg) was formulated in 10% CD + 1 HCl + NaCl. Rolipram (1, 3 and 10 mg/kg) was formulated in 10% CD + 1 HCl + NaCl. D-serine (20, 80 ...
Protein extracts from embryonic and adult hearts were obtained by mechanical disruption of the tissue using the MagNA Lyser and MagNA Lyser beads (Roche Diagnostics) in RIPA buffer (50 mM Tris-HCl, 150 mM NaCl, 1% NP-40, 0.2% sodium deoxycholate, 0.1% SDS) supplemented with protease inh...
The fresh fecal matter was diluted 1:10 in sterile PBS (10%), l-cysteine HCL (0.05%), glycerol (20%) and sterile water (60%) and passed through a 100 μm filter. The solution was frozen at −20 °C and thawed at room temperature for 12 h prior to use (the use of frozen ...
For processing of identical volumes, 0.9% sodium chloride (B. Braun, Melsungen, Germany) were used to make up a final sample volume of 8.5 mL: the sample with no heparin was diluted with 3.5 mL 0.9% NaCl, the sample with 1.5 mL heparin was diluted with 2 mL 0.9% NaCl and the ...
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Buffer solutions in the pH series 1 - 10 at unit time were organized from HCl (0.1 M) - KCl (1 M) (pH 1 and 2), CH3COOH (1 M) - NaCOOH (1 M) (pH 3 to 7), NaHCO3 (1 M)- Na2CO3 (saturated) (pH 8 and 9), and NH4Cl (1 M) -NH3solution (1 M) (pH 10). Capilla...