在此基础上,用预定量的乙醇酸来选择性地提取 16。在用 NaHCO3 水溶液和 NaCl 水溶液洗涤有机相后,在 11 的 IPAc 溶液中加入 HCl 在 i-PrOH(IPA)中的溶液,分离出产物 11 的 HCl 盐(式 2)。至此,11 的 IPAc 溶液中过量的未反应 14 被剔除在液体中。 11-HCl 的湿饼在分离过滤器中用 IPAc 进行温...
取1mLRNA提取buffer(100mMTris-HClpH8.0,50mMEDTA,pH8.0,500mMNaCl,1%SDS)于1.5mL离心管中,加入20uL的β-巯基乙醇,混匀,置于65°C保温待用。取约0.3g铁皮石斛小苗根茎叶样品,液氮下充分研磨,将粉末转移至上一步准备的RNAbuffer中,快速混匀,65°C裂解20min。12000rpm离心3min,取上清于新的离心管中。加入等...
How do you make a chemical reaction with baking soda? How much sodium bicarbonate is needed to neutralize HCl? What method is used to separate kitchen salt and water? Is potassium bicarbonate baking soda? What else is produced when sodium carbonate decomposes? Na2CO3 ? NO2O ?
LC-MS/MS analysis of protein fragments. After 5 hours HCl was added to reach pH 1.3 and the mod- ified aS was digested at 37 °C with pepsin. The amount of pepsin added was 2% (w/w) with respect to aS and the enzyme was allowed to react overnight. The same amount of ...
During the Purification of Lactate Dehydrogenase (LDH) experiment, you will need 50ml of buffer A150. Buffer A150 is 30mM Tris (pH 8.8) and 150mM NaCl. Given 1L of 1.5M Tris and 500ml of 5M NaCl, how If I have 1 liter of a 1M...
Antigen retrieval in 10 mM sodium citrate buffer, pH 6.0 for 20 min, was not followed by incubation with HCl (2 N). Incubation with the primary antibodies overnight at 4 °C was followed by incubation with 0.5 M NaCl for 6 min to eliminate nonspecific binding of the anti...
( ) (4)(2023·四川卷)20 mL 0.1 mol·L-1 CH3COONa溶液与10 mL 0.1 mol·L-1 HCl溶液混合后溶液呈酸性,所得溶液中:c(CH3COO-)>c(Cl-)>c(CH3COOH)>c(H+)。( ) (5)(2023·北京卷)测0.1 mol·L-1氨水旳pH为11:NH3·H2O≒NH+4+OH-。 ( ) (6)(2023·全国新课标Ⅱ卷)pH=5旳H2S溶液...
下列 说法错误的是 A.0.1molNH₃含有的质了数为NA B.22gN₂ . O含有的σ键数为NA C.路径①中生成11.21(已折算为标准状况)N₂ . 时,转移电 子数为NA D.25℃.11NH₂ . OH和HCl混合液中,若 ( ₃ ⁺ = ₂O ,则H⁺数目为10 −6 � 6.下列实验探究方案能达到探究目的的是 ...
“non binding surface flat bottom low flange” black 384-well plates (Corning) at room temperature in a buffer containing 20 mM TrisHCl, pH 7.5, 5 mM DTT, 100 mM NaCl, 1 mg/mL 3-[(3-cholamidopropyl) dimethylammonio] propanesulfonic acid (CHAPS) and 0.5 mg/mL bovine gamma globulin (...
Tobacco smoke (TS) contains highly reactive oxygen species (such as hydrogen peroxide, peroxynitrite, etc), which cause oxidative damage in vascular tissue and may exacerbate inflammatory events leading to the blood-brain barrier damage (BBBD) which acco