A sequence of 492 nucleotides of the amplified product was determined, which was almost superimposable onto the known sequence of the corresponding fragment of rabbit NHE 3 . It is concluded that, in rabbit gallbladder, neutral NaCl absorption is, at least in part, dependent on the NHE 3 ...
Addition of a physiological concentration of NaCl (0.9%) to 0.05 mol/l Tris buffer (pH 7.4) reversed the enhancement of the activation of glutamic-type plasminogen (Glu-Plg) by low molecular weight urokinase by fucoidan and heparin, while addition of aminohexanoic acid (6-AH) enhanced the act...
To obtain whole-cell extracts for co-IP and western blot analysis, cells were lysed using a buffer containing 40 mM HEPES, pH 7.4, 120 mM NaCl, 1 mM EDTA, 50 mM NaF, 1.5 mM Na3VO4, 10 mM β-glycerophosphate, 0.3% CHAPS (Sigma, C5849), and EDTA-free protease inhibi...
Lung tissue was homogenized in 10 mL of solution containing 10 mmol/L N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid (pH 7.9), 150 mmol/L NaCl, 1 mmol/L ethylenediamine tetraacetic acid, 0.5 mol/L phenylmethylsulfonyl fluoride, 0.6% NP-40, and a protease inhibitor cocktail (50 μL of...
Abnormal penile foreskin development in hypospadias is the most frequent genital malformation in male children, which has increased dramatically in recent decades. A number of environmental factors have been shown to be associated with hypospadias develo
Cell pellets were resuspended in LDOX lysis buffer (20 mM potassium phosphate, 200 mM NaCl, pH 7.4, 0.01 mg ml−1 DNAseI) to an OD600 of 140 and lysed by two rounds of sonication for 2 min at 50% amplitude with 2 s intervals using a Sonopuls (Bandelin) sonicator. ...
氯通道在阿霉素跨细胞膜转运中作用-role of chlorine channel in adriamycin transport across cell membrane theexpressionofendogenousClC-3chloridechannelproteinwasup-regulatedinCNE-2Zcells.TheelectrophysiologicalresultsshowedthatthechloridecurrentsinducedbyadriamycininCNE-2ZcellswerelargerthanthatinNP69-SV40Tcells.In...
Induced cells (5 g wet weight) were resuspended in 20 ml 50 mM phosphate buffer (pH 8.0) containing 300 mM NaCl, 10 mM imidazole, benzamidine/HCl, soybean trypsin inhibitor, DNase (5 μg ml−1) and RNase (10 μg ml−1). The cells were broken by one pass through a French ...
Protein Isolation and Western Blotting About 15 mm3 of orbitofrontal cortices were collected in RIPA lysis buffer (50 mM Tris-Cl, 150 mM NaCl, 1% Triton- X100, 0.5% sodium deoxycholate, 0.1% SDS, phosphatase inhibitor cocktails (04906837001; Sigma-Aldrich, Oakville, ON, Canada), protease ...
Cell-magnetic beads complex were resuspended in Dig-wash Buffer (20 mM HEPES pH 7.5; 150 mM NaCl; 0.5 mM Spermidine; 1 × Protease inhibitor cocktail; 0.05% Digitonin), and then the primary antibody of CTCF (Millipore, 07–729) was diluted 1:50 in Dig-Wash buffer and then ...