An enzyme called DNA ligase seals up the fragments into one long continuous strand. The new copies automatically wind up again. Different types of cells replicate their DNA at different rates. Some cells constantly divide, like those in your hair and fingernails and bone marrow cells. Other cell...
In addition, the ATP-dependent ligase encoded by yoqV, when cloned on a plasmid under appropriate expression signals, was unable to rescue a yerG mutant strain. The two B.subtilis ligase genes yerG and yoqV were also introduced in an Escherichia coli strain encoding a thermosensitive ligase ...
Our goal was to pinpoint the gene encoding bilirubin reductase through experimental screening and comparative genomics. The bilirubin reductase enzyme is probably an oxidoreductase that acts on carbon–carbon double bonds (Enzyme Commission (EC): 1.3.-.-; Fig.1band Extended Data Fig.1). In additi...
Male germ cell development requires precise regulation of gene activity in a cell-type and stage-specific manner, with perturbations in gene expression during spermatogenesis associated with infertility. Here, we use steady-state, nascent and single-cell
Based on the lipoate redox state, PfLipL1 either functions as a canonical lipoate ligase or as a lipoate activating enzyme which works in conjunction with PfLipL2. We demonstrate that PfLipL2 is a lipoyltransferase and is a member of a novel clade of lipoate attachment enzymes. We show...
Antagonistic control of myofiber size and muscle protein quality control by the ubiquitin ligase UBR4 during aging ArticleOpen access03 March 2021 Small heat-shock protein HSPB3 promotes myogenesis by regulating the lamin B receptor ArticleOpen access06 May 2021 ...
N6-methyladenosine (m6A) mRNA modification is essential for mammalian and plant viability. The U6 m6A methyltransferases in other species regulate S-adenosylmethionine (SAM) homeostasis through installing m6A in pre-mRNAs of SAM synthetases. However, U6
The DNA ligase from Thermus thermophilus (Tth DNA ligase) seals single-strand breaks (nicks) in DNA duplex substrates. The specificity and thermostability ... J Luo,DE Bergstrom,B Francis - 《Nucleic Acids Research》 被引量: 485发表: 1996年 Insights into DNA polymerization mechanisms from struct...
Alpha globin inhibition of eNOS-derived NO signaling necessarily requires O2, as both eNOS enzyme activity and NO scavenging and subsequent transformation to NO3– by alpha globin require O2. Conversely, endothelial alpha globin (as with erythrocyte hemoglobin) must be deoxygenated to act as a ...
Benzaldehyde, the simplest aromatic aldehyde, is one of the most wide-spread volatiles that serves as a pollinator attractant, flavor, and antifungal compound. However, the enzyme responsible for its formation in plants remains unknown. Using a combinati