We describe a strategy whereby the activity of a ubiquitin ligase has been enzymatically reversed, accomplished by fusing it to a catalytic domain of an exogenous deubiquitinating enzyme. We present a library of 72 "anti-ligases" that appear to work in a dominant-negative fashion to stabilize ...
An RNA ligase activity has previously been detected in extracts of E. coli which is capable of joining S. cerevisiae tRNA splicing intermediates in the absence of ATP to form a 2'-5'phosphodiester linkage (36). In order to study the mechanism and function of this unusual enzyme in bacterial...
The joining of Okazaki fragments during lagging strand DNA replication in mammalian cells is believed to be due to DNA ligase I. This enzyme is composed of a 78-kDa carboxyl-terminal catalytic domain and a 24-kDa amino-terminal region that is not required for ligation activity in vitro. ...
Here, we use multiscale modelling of three Escherichia coli enzymes (type III chloramphenicol acetyltransferase, d-alanine–d-alanine ligase B and dihydrofolate reductase) to understand experimentally measured changes in specific activity due to synonymous mutations. The modelling involves coarse-grained ...
Small RNA biogenesis: novel roles of an RNase III enzyme. Nat. Plants 2, 16021 (2016). Article CAS PubMed Google Scholar Ausin, I., Greenberg, M. V., Li, C. F. & Jacobsen, S. E. The splicing factor SR45 affects the RNA-directed DNA methylation pathway in Arabidopsis. Epigenetics...
VIRUS DNA-LIGASEWHEAT-GERMPOLYNUCLEOTIDE KINASECRYSTAL-STRUCTURECAPPING ENZYMETrill is an essential 827-amino-acid enzyme that executes the end-healing and end-sealing steps of tRNA splicing in Saccharomyces cerevisiae. Trl1 consists of two catalytic domains-an N-terminal adenylyltransferase/ligase ...
The next day, the cells were subjected to experimental analysis or transplanted into the ovarian bursa of mice. Enzyme-linked immunosorbent assay (ELISA) Following cell counting, human UC-MSCs were seeded in 6-well plates at the same cell density. After 24 h of incubation, the supernatant was...
To overcome common drawbacks of conventional methods, we exploited the unique catalytic properties of an artificial enzyme, ribozyme ligase, to develop a selection methodology in which the entire detection process takes place in a homogeneous solution, thus eliminating the need for affinity separation. ...
et al. Crystal structure of UDP-N-acetylmuramoyl-l-alanyl-d-glutamate: meso-diaminopimelate ligase from Escherichia coli. J. Biol. Chem. 276, 10999–11006 (2001). Article CAS PubMed Google Scholar Smith, C. A. Structure, function and dynamics in the mur family of bacterial cell wall ...
RNase T1 cleaves single-stranded RNA 3' to guanosine residues. Partial digestion of 3' or 5' labeled RNA with this enzyme thus generates a ladder of G residues. The 1X RNA Sequencing Buffer used in the procedure contains 7 M Urea to help denature RNA...