a, Gene tree constructed from putativebilRsequences and related reductases from the Old Yellow Enzyme family. Experimentally confirmed bilirubin reducers are labelled in the tree. Clade 1 indicates the overallbilRclade, and clade 2 indicates the shortbilRclade. The same tree with all bootstrap v...
mutations abolish the methyltransferase activity [32,45]. Based on the sequence alignments among multiple species (Additional file1: Fig. S1b), we designed an inactive FIONA1 P237A/F239G with mutations of putative catalytic ligand NPPF and named as FIONA1m. To further confirm that U6 snRNA i...
Functional activity of N-methyl-D-aspartate (NMDA) receptors requires both glutamate binding and the binding of an endogenous coagonist that has been presumed to be glycine, although D-serine is a more potent agonist. Localizations of D-serine and it biosynthetic enzyme serine racemase approximate...
Using a seven-step biochemical fractionation procedure and a drop-out mass spectrometry approach, we show that a previously undescribed biomolecule, S-geranylgeranyl-l-glutathione (GGG), is a potent P2RY8 ligand that is detectable in lymphoid tissues at the nanomolar level. GGG inhibited the ...
Each type of tissue sample was collected from nine individuals as an independent biological replicate for transcriptome sequencing. To minimize biological variance, three independent biological replicates were performed for each tissue sample. All tissue samples were immediately placed into enzyme-free ...
Galantamine (Reminyl<sup>TM</sup>) is a novel drug treatment for mild to moderate Alzheimer's disease (AD). Originally established as a reversible inhibitor of the acetylcholine-degrading enzyme acetylcholinesterase (AChE), galantamine also acts as an allosterically potentiating ligand (APL) on nicot...
The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor residing in the cytoplasm in the form of a multiprotein complex. Following ligand binding, the receptor dissociates from the complex and translocates into the nucleus where it initiates transcription of various genes (in ...
Metformin, a type 2 diabetes treatment, improves the cognitive function of aged mice; however, whether the protective effects of metformin on cognitive function in aged mice are associated with the gut microbiome is poorly understood. Although some studi
Triple-negative breast cancer (TNBC) has a poor clinical outcome, due to a lack of actionable therapeutic targets. Herein we define lysosomal acid lipase A (LIPA) as a viable molecular target in TNBC and identify a stereospecific small molecule (ERX-41) that binds LIPA. ERX-41 induces endopl...
3D structures of GH3 enzymes indicated the presence of an additional acid residue in the active site (Fig. 1b). This residue is conserved in at least 200 members of the family and directed mutagenesis studies revealed a measurable effect on enzyme activity30,31, which led to suggest that it...