It has previously been established that dGTP acts as both an activator and a substrate of this enzyme, suggesting that phosphohydrolase activity of SAMHD1 is regulated by dGTP availability in the cell. However, we now demonstrate biochemically that the NTP GTP is equally capable of activating SAM...
Gamma glutamyl transpeptidase (GGT) is a membrane bound enzyme that plays a key role in the synthesis of the antioxidant glutathione. Epidemiological studies have linked high GGT with an increased risk of morbidity and cardiovascular mortality. In contrast, GGT is usually elevated in liver transplant...
It has previously been established that dGTP acts as both an activator and a substrate of this enzyme, suggesting that phosphohydrolase activity of SAMHD1 is regulated by dGTP availability in the cell. However, we now demonstrate biochemically that the NTP GTP is equally capable of activating SAM...
In C. perfringens, SleC acts as an SCLE with both lytic transglycosylase and amidase activities [251]. Premature activation is controlled by expressing the enzyme as a propeptide dependent on the Csp protease complex after ion release and partial [257,258,264]. The second class of GSLEs is ...
The closely related small GTP-binding proteins Rac1, Rac2, and Rac3 are part of a larger Rho subfamily of Ras proteins. Because disruption of Ras signaling pathways is relevant to the pathogenesis of a wide variety of cancers, it is important to clearly define the structural and functional ch...
[1]. For it to qualify, the physiological level of the hormonal signal must activate membrane guanylate cyclase residing in the plasma membrane portion of the cell. Guanylate cyclase is an enzyme that catalyzes the transformation of GTP into cyclic GMP. Based, at the time, on the only ...
Activation of SAMHD1 phosphohydrolase activity was tested under physiological concentrations of dGTP or GTP found in either dividing or non-dividing cells. Because GTP is 1000-fold more abundant than dGTP in cells, GTP was able to activate the enzyme to a greater extent than dGTP, suggesting ...
falciparum imports the host lipid-peroxidation repair enzyme PRDX6 during blood stage, allowing it to repair lipid-peroxidation damage. Moreover, we show that PRDX6 also plays an important role in the transport of HCVs to the FV. HCCU is a...
METTL3 is an essential m6A regulator enzyme in colorectal carcinogenesis. Functionally, METTL3 promotes CRC initiation and progression through inducing a novel m6A-GLUT1-mTORC1 axis. Conserved motifs (DPPW, IHM and RTGRTGH) among MTase domain are important for METTL3 function in CRC. Inhibition ...
2d). Importantly, the activity of Xrn1 on 5′P RNA was 20 fold more efficient than NAD-capped RNA at comparable enzyme concentrations (Fig. 2d and Supplementary Fig. 2d). Furthermore, Xrn1 degraded the NAD-capped RNA twice as efficiently as Rat1. To assess whether Xrn1 hydrolyzed the ...