Subject areas Biochemistry Molecular biology Biophysics Bioinformatics Introduction Heterotrimeric G proteins are a family of guanine nucleotide-binding proteins composed of three distinct subunits (Gα, Gβ, and Gγ). G proteins exist in inactive or active states depending on whether the nucleotide boun...
Download: Download full-size image Figure 2. RanGTP regulates nuclear translocation of KAP3. A, the dominant-negative form of Ran blocked nuclear localization of KAP3 in COS-7 cells. mCherry-tagged wildtype Ran or GTP-bound dominant mutant RanQ69L was cotransfected with enhanced GFP (EGFP)...
also found in the yeast protein Ysl2p [28], all of them are unique to these two ArfGEFs subfamilies within the detection limits of the BLAST search. The HUS domain features a remarkably conserved N(Y/F)DC(D/N) motif, which we call the HUS box, which is predicted to locate in a l...
Further, the caspase-3 resistant TIAM1 construct C1199DN, a stable guanine exchange factor able to constitutively activate Rac1 in the form of Rac1路GTP, and constitutively active Rac1路GTP also rescues the siRNA-caused dendritic spine deficits, respectively. These results implicate Sig-1Rs as ...
Full size image (A) GTP binding by wild type and GTP binding dead mutated HA-tagged DAPK1, showing input lysates (lower panel) and DAPK1 precipitated by binding to GTP-agarose beads.The T701N mutated form of DAPK1 was unable to bind GTP-agarose. The interaction of wild type DAPK1 with...
Biochemistry DNA Enzyme mechanisms Structural biology This article is cited by Molecular basis for transposase activation by a dedicated AAA+ ATPase Álvaro de la Gándara Mercedes Spínola-Amilibia Ernesto Arias-Palomo Nature(2024) Zinc-finger BED domains drive the formation of the active Hermes tra...
At all three fusion proteins, GTP, ITP, and XTP exhibited unique profiles with respect to their potency and efficacy at disrupting high-affinity agonist binding and supporting adenylyl cyclase activation by partial and full agonists. Our data can be interpreted in two ways: (i) GTP, ITP, and...
E Jacquet,S Baouz,A Parmeggiani - 《Biochemistry》 被引量: 73发表: 1995年 Properties of the catalytic domain of CDC25, a Saccharomyces cerevisiae GDP/GTP exchange factor: comparison of its activity on full-length and C-terminal t... Two C-terminal fragments (334 and 509 amino acid resid...
Full size image In order to test whether the cytoophidia in iPSCs respond to an increase in GTP level, cells were treated with 1 mM guanosine. Having entered the cell, the guanosine would be converted into guanine by purine nucleoside phosphorylase (PNP), then into GMP by hypoxanthine guanine...
Lochrie MA, Simon MI: G protein multiplicity in eukaryotic signal transduction systems. Biochemistry 27: 4957–4965 (1988). PubMedGoogle Scholar Logothetis DE, Kurachi Y, Galper J, Neer EJ, Clapham DE: The βγ subunits of GTP-binding proteins activate the muscarinic K+channel in heart. Natu...