The nucleotide derivatives of guanine perform important functions in cellular metabolism. GTP acts as acoenzymein carbohydrate metabolism and in the biosynthesis of proteins; it can readily donate one of its phosphate groups to adenosine diphosphate (ADP) to formadenosine triphosphate(ATP), an ...
Download: Download full-size image Figure 2. RanGTP regulates nuclear translocation of KAP3. A, the dominant-negative form of Ran blocked nuclear localization of KAP3 in COS-7 cells. mCherry-tagged wildtype Ran or GTP-bound dominant mutant RanQ69L was cotransfected with enhanced GFP (EGFP)...
Download: Download full-size image Fig. 2. Effect of heat (A) and trypsin (B) treatment of CCV on the GDP dissociation activity on Rab5. A, CCV were first incubated at 95 °C for 3 min (lane 2) or 56 °C for 10 min (lane 3) or 20 min (lane 4) before assaying for stimul...
86-01-1 (acid form); 36051-31-7 (3Na salt) Formula C10H13N5Na3O14P3 Molecular weight 589.18 g/moL (acid form: 523.18 g/moL) Purity(HPLC) ≥ 99% Content 100 mM ± 5 mM Structure Shipping and Storage The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ fo...
Full size image GTP-generating reaction of DYNAMO1 induces membrane fission To understand molecular function of DYNAMO1 on the MD and POD machinery, genetic disruption of DYNAMO1, particularly abolishment of nucleoside diphosphate kinase activity is needed. Nucleotide diphosphate kinases form an oligomer...
in rats. This previously undescribed form of endocytosis could be slow (tens of seconds) and/or rapid (a few seconds), similar to GTP- and dynamin-dependent endocytosis. It was activated during intense stimulation, whereas GTP- and dynamin-dependent endocytosis dominated during mild stimulation. ...
American Society for Biochemistry and Molecular BiologyJournal of Biological ChemistryUthaiah,R.C,G.J.Praefcke,J.C.Howard. ⅡGP1,an interferon-gamma-inducible 47-kDa GTPase of the mouse,showing cooperative enzymatic activity and GTP-dependent multimerization[J].Journal of Biological Chemistry,2003...
Full size table Evolution of BIGs and GFBs from a common ancestor Combined, our analysis reveals that the BIG and GBF subfamilies share the same overall domain organization, and are likely to descend from a common ancestor gene that duplicated first to form the BIG and GBF groups, and again...
At all three fusion proteins, GTP, ITP, and XTP exhibited unique profiles with respect to their potency and efficacy at disrupting high-affinity agonist binding and supporting adenylyl cyclase activation by partial and full agonists. Our data can be interpreted in two ways: (i) GTP, ITP, and...
Given the preferential association of GDP/GTP exchange factors with the nucleotide-free form of small GTPases, these observations raised the possibility that the complex may exert nucleotide exchange activity on Rab5. Download: Download high-res image (172KB) Download: Download full-size image ...